| pubmed-article:20453073 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20453073 | lifeskim:mentions | umls-concept:C0001774 | lld:lifeskim |
| pubmed-article:20453073 | lifeskim:mentions | umls-concept:C1000590 | lld:lifeskim |
| pubmed-article:20453073 | lifeskim:mentions | umls-concept:C0085345 | lld:lifeskim |
| pubmed-article:20453073 | lifeskim:mentions | umls-concept:C1022708 | lld:lifeskim |
| pubmed-article:20453073 | lifeskim:mentions | umls-concept:C0013126 | lld:lifeskim |
| pubmed-article:20453073 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:20453073 | lifeskim:mentions | umls-concept:C0175721 | lld:lifeskim |
| pubmed-article:20453073 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:20453073 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:20453073 | pubmed:dateCreated | 2010-7-7 | lld:pubmed |
| pubmed-article:20453073 | pubmed:abstractText | In the bipolar basidiomycete Pholiota microspora, a pair of homeodomain protein genes located at the A-mating-type locus regulates mating compatibility. In the present study, we used a DNA-mediated transformation system in P. microspora to investigate the homeodomain proteins that control the clamp formation. When a single homeodomain protein gene (A3-hox1 or A3-hox2) from the A3 monokaryon strain was transformed into the A4 monokaryon strain, the transformants produced many pseudoclamps but very few clamps. When two homeodomain protein genes (A3-hox1 and A3-hox2) were transformed either separately or together into the A4 monokaryon, the ratio of clamps to the clamplike cells in the transformants was significantly increased to ca. 50%. We therefore concluded that the gene dosage of homeodomain protein genes is important for clamp formation. When the sip promoter was connected to the coding region of A3-hox1 and A3-hox2 and the fused fragments were introduced into NGW19-6 (A4), the transformants achieved more than 85% clamp formation and exhibited two nuclei per cell, similar to the dikaryon (NGW12-163 x NGW19-6). The results of real-time reverse transcription-PCR confirmed that sip promoter activity is greater than that of the native promoter of homeodomain protein genes in P. microspora. Thus, we concluded that nearly 100% clamp formation requires high expression levels of homeodomain protein genes and that altered expression of the A-mating-type genes alone is sufficient to drive true clamp formation. | lld:pubmed |
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| pubmed-article:20453073 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20453073 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20453073 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20453073 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20453073 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20453073 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:20453073 | pubmed:issn | 1535-9786 | lld:pubmed |
| pubmed-article:20453073 | pubmed:author | pubmed-author:AimiTadanoriT | lld:pubmed |
| pubmed-article:20453073 | pubmed:author | pubmed-author:MukaiyamaHiro... | lld:pubmed |
| pubmed-article:20453073 | pubmed:author | pubmed-author:TachikawaTaka... | lld:pubmed |
| pubmed-article:20453073 | pubmed:author | pubmed-author:ShimomuraNori... | lld:pubmed |
| pubmed-article:20453073 | pubmed:author | pubmed-author:YiRuirongR | lld:pubmed |
| pubmed-article:20453073 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20453073 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:20453073 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20453073 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20453073 | pubmed:pagination | 1109-19 | lld:pubmed |
| pubmed-article:20453073 | pubmed:dateRevised | 2011-7-19 | lld:pubmed |
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| pubmed-article:20453073 | pubmed:meshHeading | pubmed-meshheading:20453073... | lld:pubmed |
| pubmed-article:20453073 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20453073 | pubmed:articleTitle | A-mating-type gene expression can drive clamp formation in the bipolar mushroom Pholiota microspora (Pholiota nameko). | lld:pubmed |
| pubmed-article:20453073 | pubmed:affiliation | Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan. | lld:pubmed |
| pubmed-article:20453073 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20453073 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20453073 | lld:pubmed |
