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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2010-7-7
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pubmed:abstractText |
In the bipolar basidiomycete Pholiota microspora, a pair of homeodomain protein genes located at the A-mating-type locus regulates mating compatibility. In the present study, we used a DNA-mediated transformation system in P. microspora to investigate the homeodomain proteins that control the clamp formation. When a single homeodomain protein gene (A3-hox1 or A3-hox2) from the A3 monokaryon strain was transformed into the A4 monokaryon strain, the transformants produced many pseudoclamps but very few clamps. When two homeodomain protein genes (A3-hox1 and A3-hox2) were transformed either separately or together into the A4 monokaryon, the ratio of clamps to the clamplike cells in the transformants was significantly increased to ca. 50%. We therefore concluded that the gene dosage of homeodomain protein genes is important for clamp formation. When the sip promoter was connected to the coding region of A3-hox1 and A3-hox2 and the fused fragments were introduced into NGW19-6 (A4), the transformants achieved more than 85% clamp formation and exhibited two nuclei per cell, similar to the dikaryon (NGW12-163 x NGW19-6). The results of real-time reverse transcription-PCR confirmed that sip promoter activity is greater than that of the native promoter of homeodomain protein genes in P. microspora. Thus, we concluded that nearly 100% clamp formation requires high expression levels of homeodomain protein genes and that altered expression of the A-mating-type genes alone is sufficient to drive true clamp formation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-10441440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-12242384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-1353886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-1369739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-14643262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-15157509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-15879506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-16096791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-16461425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-17248739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-18557817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-19049868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-216315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-6336730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-6353143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-7489716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-7598694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-7720075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-7913746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-7994179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-8242751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-8821660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-9178005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20453073-9539426
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1535-9786
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1109-19
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pubmed:dateRevised |
2011-7-19
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pubmed:meshHeading |
pubmed-meshheading:20453073-Cell Nucleus,
pubmed-meshheading:20453073-Fungal Proteins,
pubmed-meshheading:20453073-Gene Expression Regulation, Fungal,
pubmed-meshheading:20453073-Genes, Mating Type, Fungal,
pubmed-meshheading:20453073-Homeodomain Proteins,
pubmed-meshheading:20453073-Indoles,
pubmed-meshheading:20453073-Pholiota,
pubmed-meshheading:20453073-Physical Chromosome Mapping,
pubmed-meshheading:20453073-Polymerase Chain Reaction,
pubmed-meshheading:20453073-Protein Binding,
pubmed-meshheading:20453073-Transcription, Genetic,
pubmed-meshheading:20453073-Transformation, Genetic,
pubmed-meshheading:20453073-Two-Hybrid System Techniques
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pubmed:year |
2010
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pubmed:articleTitle |
A-mating-type gene expression can drive clamp formation in the bipolar mushroom Pholiota microspora (Pholiota nameko).
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pubmed:affiliation |
Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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