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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1991-7-18
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pubmed:abstractText |
A heat-stable enterotoxin produced by Vibrio mimicus (VM-ST) was studied. VM-ST was purified from a culture supernatant of V. mimicus strain AQ-0915 by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatography on both SP-Sephadex C-50 and DEAE-Sephadex A-25, and HPLC, and the recovery rate was about 15%. Purified VM-ST was heat-stable. VM-ST activity was cross-neutralized by anti-STh antiserum. The amino acid composition of the purified VM-ST was determined 17 amino acid residues in the following sequence: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This composition and sequence were identical to those of V. cholerae non-O1-ST. These results clearly demonstrate the production of a characteristic VM-ST by V. mimicus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0378-1097
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
105-10
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2044934-Amino Acid Sequence,
pubmed-meshheading:2044934-Amino Acids,
pubmed-meshheading:2044934-Chromatography,
pubmed-meshheading:2044934-Chromatography, High Pressure Liquid,
pubmed-meshheading:2044934-Enterotoxins,
pubmed-meshheading:2044934-Kinetics,
pubmed-meshheading:2044934-Molecular Sequence Data,
pubmed-meshheading:2044934-Neutralization Tests,
pubmed-meshheading:2044934-Sequence Alignment,
pubmed-meshheading:2044934-Temperature,
pubmed-meshheading:2044934-Vibrio
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pubmed:year |
1991
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pubmed:articleTitle |
Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus.
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pubmed:affiliation |
Research Institute for Microbial Diseases, Osaka University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|