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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 5
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pubmed:dateCreated |
2010-5-6
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pubmed:abstractText |
AlgX is a periplasmic protein required for the production of the exopolysaccharide alginate in Pseudomonas sp. and Azotobacter vinelandii. AlgX has been overexpressed and purified and diffraction-quality crystals have been grown using iterative seeding and the hanging-drop vapor-diffusion method. The crystals grew as flat plates with unit-cell parameters a = 46.4, b = 120.6, c = 86.9 A, beta = 95.7 degrees . The crystals exhibited the symmetry of space group P2(1) and diffracted to a minimum d-spacing of 2.1 A. On the basis of the Matthews coefficient (V(M) = 2.25 A(3) Da(-1)), two molecules were estimated to be present in the asymmetric unit.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20445266-10801485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20445266-10867591,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20445266-9710677
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1744-3091
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
588-91
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pubmed:dateRevised |
2011-7-28
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pubmed:meshHeading |
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pubmed:year |
2010
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pubmed:articleTitle |
Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX.
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pubmed:affiliation |
Program in Molecular Structure and Function, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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