Linked Life Data

20443705

Source:http://linkedlifedata.com/resource/pubmed/id/20443705

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-5-6
pubmed:abstractText
Internalization of monoclonal antibodies (MAbs) binding to the targeted cells has drawn great attention to both scientists and anti-cancer drug developmental professionals. Internalization of conjugated MAb is thought to be one of the major mechanisms for tumor cell destruction, and can be studied using several biochemical and microscopic approaches. Here we report a new method based on papain digestion followed by flow cytometry (FCM). This method can identify whether the binding MAb has internalized into the cell, with an additional advantage of accurately quantifying the internalized MAb without altering cell morphology after papain digestion. With this method, we studied the internalization degrees of 3A4 (a mouse anti-human CD45RA MAb) at different time points: 5.3% (15 min), 7.3% (30 min), 36.9% (60 min), 69.2% (120 min), and 72.6% (180 min). This methodology can facilitate our understanding of the efficiency of MAb internalization and allows us to evaluate the targeted killing capacity of the MAb. Our technique can serve as a reference model for future targeted drug development using MAbs. In summary, we established a simple and useful evaluation tool for MAb drug development and research.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1557-8348
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Accurate determination of internalization for target binding antibody using papain digestion and flow cytometry.
pubmed:affiliation
Division of Hematology-Oncology, Zhejiang Key Laboratory for Neonatal Disease, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't