Linked Life Data

20436

Source:http://linkedlifedata.com/resource/pubmed/id/20436

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1977-11-30
pubmed:abstractText
Streptomyces nogalater, UC-2783, and Streptomyces peucetius var. caesius, IMRU-3920/UC-5633, catalyze ketonic carbonyl reduction of steffimycinone (1, Scheme 1). Using cell-free preparations of S. nogalater, the process of ketonic carbonyl reduction has been shown to be TPNH linked. The product, steffimycinol (2), is reduced further by Aeromonas hydrophila, 2C/UC-6303, by the process of microaerophilic conversion of anthracyclinones previously reported1,2) with the result being the formation of 7-deoxysteffimycinol (3). The products (2 and 3) were isolated by extraction from the fermentations followed by chromatographic purification. Identification was by comparison of various physical properties and spectral data with those of authentic materials obtained by chemical means. Catalytic activity of the crude enzyme preparations of S. nogalater was lost by dialysis by restored by addition of TPNH although not by addition of DPNH demonstrating TPNH dependence. The reaction rate increased linearly with added crude enzyme protein up to 4 mg/ml and was highest between pH 6.5 and 7.0.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-8820
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
649-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Bacterial metabolism of anthracycline antibiotics. Steffimycinone and steffimycinol conversions.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.