| pubmed-article:20433876 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20433876 | lifeskim:mentions | umls-concept:C0299583 | lld:lifeskim |
| pubmed-article:20433876 | lifeskim:mentions | umls-concept:C0005953 | lld:lifeskim |
| pubmed-article:20433876 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:20433876 | lifeskim:mentions | umls-concept:C1257975 | lld:lifeskim |
| pubmed-article:20433876 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:20433876 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:20433876 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:20433876 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:20433876 | pubmed:issue | 1-3 | lld:pubmed |
| pubmed-article:20433876 | pubmed:dateCreated | 2010-7-12 | lld:pubmed |
| pubmed-article:20433876 | pubmed:abstractText | Previous studies demonstrate that leptin has an osteogenic differentiation effect on bone marrow mesenchymal stem cells (MSCs). However, the effect of adenovirus-mediated leptin on MSCs differentiation has not been reported. To explore the mechanism, we constructed a recombinant adenoviral vector Ad-leptin and transfected propagated MSCs in vitro. The effects of Ad-leptin on MSCs growth and osteogenic differentiation were examined. The results showed that Ad-leptin inhibited the transfected MSCs growth significantly, and up-regulated osteocalcin expression and alkaline phosphatase activity. The expression of Cbfalpha1 and Cbfbeta which were the key factors in osteogenic differentiation was also up-regulated. All the findings suggest that genetic engineering of MSCs to express leptin gene may have potential application in the treatment of several genetic diseases and bone reconstruction. | lld:pubmed |
| pubmed-article:20433876 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20433876 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20433876 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20433876 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20433876 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20433876 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20433876 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20433876 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:20433876 | pubmed:issn | 1873-1686 | lld:pubmed |
| pubmed-article:20433876 | pubmed:author | pubmed-author:LiuJianminJ | lld:pubmed |
| pubmed-article:20433876 | pubmed:author | pubmed-author:ZhangYuhuiY | lld:pubmed |
| pubmed-article:20433876 | pubmed:author | pubmed-author:YueZhijianZ | lld:pubmed |
| pubmed-article:20433876 | pubmed:author | pubmed-author:JingYingyingY | lld:pubmed |
| pubmed-article:20433876 | pubmed:author | pubmed-author:XiaowuHuH | lld:pubmed |
| pubmed-article:20433876 | pubmed:author | pubmed-author:LaixingWangW | lld:pubmed |
| pubmed-article:20433876 | pubmed:author | pubmed-author:JinchuanLiang... | lld:pubmed |
| pubmed-article:20433876 | pubmed:author | pubmed-author:HanGuoshengG | lld:pubmed |
| pubmed-article:20433876 | pubmed:copyrightInfo | Copyright (c) 2010 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:20433876 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20433876 | pubmed:day | 9 | lld:pubmed |
| pubmed-article:20433876 | pubmed:volume | 163 | lld:pubmed |
| pubmed-article:20433876 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20433876 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20433876 | pubmed:pagination | 107-12 | lld:pubmed |
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| pubmed-article:20433876 | pubmed:meshHeading | pubmed-meshheading:20433876... | lld:pubmed |
| pubmed-article:20433876 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20433876 | pubmed:articleTitle | Osteogenic differentiation of bone marrow mesenchymal stem cells by adenovirus-mediated expression of leptin. | lld:pubmed |
| pubmed-article:20433876 | pubmed:affiliation | Department of Neurosurgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. | lld:pubmed |
| pubmed-article:20433876 | pubmed:publicationType | Journal Article | lld:pubmed |
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