|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-3
|
|
pubmed:dateCreated |
2010-7-12
|
|
pubmed:abstractText |
Previous studies demonstrate that leptin has an osteogenic differentiation effect on bone marrow mesenchymal stem cells (MSCs). However, the effect of adenovirus-mediated leptin on MSCs differentiation has not been reported. To explore the mechanism, we constructed a recombinant adenoviral vector Ad-leptin and transfected propagated MSCs in vitro. The effects of Ad-leptin on MSCs growth and osteogenic differentiation were examined. The results showed that Ad-leptin inhibited the transfected MSCs growth significantly, and up-regulated osteocalcin expression and alkaline phosphatase activity. The expression of Cbfalpha1 and Cbfbeta which were the key factors in osteogenic differentiation was also up-regulated. All the findings suggest that genetic engineering of MSCs to express leptin gene may have potential application in the treatment of several genetic diseases and bone reconstruction.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1873-1686
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright (c) 2010 Elsevier B.V. All rights reserved.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
9
|
|
pubmed:volume |
163
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
107-12
|
|
pubmed:meshHeading |
pubmed-meshheading:20433876-Adenoviridae,
pubmed-meshheading:20433876-Alkaline Phosphatase,
pubmed-meshheading:20433876-Cell Differentiation,
pubmed-meshheading:20433876-Cell Proliferation,
pubmed-meshheading:20433876-Cells, Cultured,
pubmed-meshheading:20433876-Core Binding Factor Alpha 1 Subunit,
pubmed-meshheading:20433876-Core Binding Factor beta Subunit,
pubmed-meshheading:20433876-Genetic Engineering,
pubmed-meshheading:20433876-Genetic Vectors,
pubmed-meshheading:20433876-Humans,
pubmed-meshheading:20433876-Leptin,
pubmed-meshheading:20433876-Mesenchymal Stem Cells,
pubmed-meshheading:20433876-Osteocalcin,
pubmed-meshheading:20433876-Recombinant Fusion Proteins,
pubmed-meshheading:20433876-Up-Regulation
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Osteogenic differentiation of bone marrow mesenchymal stem cells by adenovirus-mediated expression of leptin.
|
|
pubmed:affiliation |
Department of Neurosurgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
|
|
pubmed:publicationType |
Journal Article
|
