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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2010-5-20
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pubmed:abstractText |
Thymocytes are highly motile cells that migrate under the influence of chemokines in distinct thymic compartments as they mature. The motility of thymocytes is tightly regulated; however, the molecular mechanisms that control thymocyte motility are not well understood. Here we report that G protein-coupled receptor kinase-interactor 2 (GIT2) was required for efficient positive selection. Notably, Git2(-/-) double-positive thymocytes showed greater activation of the small GTPase Rac, actin polymerization and migration toward the chemokines CXCL12 (SDF-1) and CCL25 in vitro. By two-photon laser-scanning microscopy, we found that the scanning activity of Git2(-/-) thymocytes was compromised in the thymic cortex, which suggests GIT2 has a key role in regulating the chemokine-mediated motility of double-positive thymocytes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Ccl25 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Git2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Rac1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/rac GTP-Binding Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1529-2916
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
503-11
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pubmed:meshHeading |
pubmed-meshheading:20431621-Animals,
pubmed-meshheading:20431621-Apoptosis,
pubmed-meshheading:20431621-Calcium,
pubmed-meshheading:20431621-Cell Cycle Proteins,
pubmed-meshheading:20431621-Cell Movement,
pubmed-meshheading:20431621-Cells, Cultured,
pubmed-meshheading:20431621-Chemokine CXCL12,
pubmed-meshheading:20431621-Chemokines, CC,
pubmed-meshheading:20431621-Down-Regulation,
pubmed-meshheading:20431621-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:20431621-Fluorescent Antibody Technique,
pubmed-meshheading:20431621-Mice,
pubmed-meshheading:20431621-Mice, Inbred BALB C,
pubmed-meshheading:20431621-Mice, Inbred C57BL,
pubmed-meshheading:20431621-Mice, Transgenic,
pubmed-meshheading:20431621-Neuropeptides,
pubmed-meshheading:20431621-Phosphoproteins,
pubmed-meshheading:20431621-Selection, Genetic,
pubmed-meshheading:20431621-Thymus Gland,
pubmed-meshheading:20431621-rac GTP-Binding Proteins
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pubmed:year |
2010
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pubmed:articleTitle |
Regulation of thymocyte positive selection and motility by GIT2.
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pubmed:affiliation |
Department of Medicine, University of California San Francisco, San Francisco, CA, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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