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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2010-5-27
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pubmed:abstractText |
There are no ideal ways to identify and isolate viable and purified Foxp3(+) regulatory T cells so far. Here we developed a novel procedure for the isolation of highly purified Foxp3(+) cells using flow cytometry. This method relies on an identification and sorting of the lymphoblast cell population identified on a scatter plot using flow cytometry. We confirmed that greater than 98% of the cells sorted using this technique expressed Foxp3 and displayed a potent suppressive activity. This method provides a valuable tool for the study of the T regulatory cell biology and their therapeutic manipulation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1759-4685
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
164-9
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pubmed:dateRevised |
2010-10-21
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pubmed:meshHeading |
pubmed-meshheading:20430855-Animals,
pubmed-meshheading:20430855-Cell Separation,
pubmed-meshheading:20430855-Female,
pubmed-meshheading:20430855-Flow Cytometry,
pubmed-meshheading:20430855-Forkhead Transcription Factors,
pubmed-meshheading:20430855-Gene Knock-In Techniques,
pubmed-meshheading:20430855-Green Fluorescent Proteins,
pubmed-meshheading:20430855-Interleukin-2 Receptor alpha Subunit,
pubmed-meshheading:20430855-Mice,
pubmed-meshheading:20430855-Mice, Inbred C57BL,
pubmed-meshheading:20430855-T-Lymphocytes, Regulatory
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pubmed:year |
2010
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pubmed:articleTitle |
Isolation of purified and live Foxp3+ regulatory T cells using FACS sorting on scatter plot.
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pubmed:affiliation |
Division of Rheumatology and Immunology, Department of Medicine, University of Southern California, Los Angeles, CA, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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