Linked Life Data

20424635

Source:http://linkedlifedata.com/resource/pubmed/id/20424635

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2010-12-1
pubmed:abstractText
The innate immune response is involved in the pathophysiology of cerebral ischemia-reperfusion (I/R) injury. Recent evidence suggests that scavenger receptors have a role in the induction of innate immunity. In this study, we examined the role of scavenger receptor A (SR-A) in focal cerebral I/R injury. Both SR-A(-/-) mice (n=10) and age-matched wild-type (WT) mice (n=9) were subjected to focal cerebral ischemia (60?minutes), followed by reperfusion (for 24?hours). Infarct size was determined by TTC (triphenyltetrazolium chloride) staining. The morphology of neurons in the brain sections was examined by Nissl's staining. Activation of intracellular signaling was analyzed by western blot. Cerebral infarct size in SR-A(-/-) mice was significantly reduced by 63.9% compared with WT mice after cerebral I/R. In SR-A(-/-) mice, there was less neuronal damage in the hippocampus compared with WT mice. Levels of FasL, Fas, FADD, caspase-3 activity, and terminal deoynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling-positive apoptotic cells were significantly increased in WT mice after cerebral I/R, but not in SR-A(-/-) mice. Cerebral I/R increased nuclear factor-?B activation in WT mice, but not in SR-A(-/-) mice. These data suggest that SR-A has a central role in cerebral I/R injury and that suppression of SR-A may be a useful approach for ameliorating brain injury in stroke patients.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1559-7016
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1972-81
pubmed:dateRevised
2010-12-20
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Scavenger receptor class-A has a central role in cerebral ischemia-reperfusion injury.
pubmed:affiliation
Department of Surgery, East Tennessee State University, Johnson City, Tennessee, USA. Li@etsu.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural