Linked Life Data

20423331

Source:http://linkedlifedata.com/resource/pubmed/id/20423331

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-6-15
pubmed:abstractText
snoRNAs (small nucleolar RNAs) are key components of snoRNP (small nucleolar ribonucleoprotein) particles involved in modifying specific residues of ribosomal and other RNAs by pseudouridylation (H/ACA snoRNAs) or methylation (C/D snoRNAs). They are encoded within the introns of host genes, which tend to be genes whose products are involved in ribosome biogenesis or function. Although snoRNPs are abundant, ubiquitous and their components highly conserved, information concerning their expression during development or how their expression is altered in diseased states is sparse. To facilitate these studies we have developed a snoRNA microarray platform for the analysis of the abundance of snoRNAs in different RNA samples. In the present study we show that the microarray is sensitive and specific for the detection of snoRNAs. A mouse snoRNA microarray was used to monitor changes in abundance of snoRNAs after ablation of dyskerin, an H/ACA snoRNA protein component, from mouse liver, which causes a decrease in ribosome production. H/ACA snoRNAs were decreased in abundance in these livers while, unexpectedly, C/D snoRNAs were increased. The increase in C/D snoRNAs corresponded with an increase in the abundance of the mRNAs transcribed from snoRNA host genes, suggesting the increase may be part of a cellular response to defective ribosome synthesis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-11074001, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-11850781, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-12007400, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-12400016, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-12457565, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-12470739, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-12612653, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-15574827, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-15755954, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-15770508, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-15974869, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-17381323, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-19770060, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-19796927, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-19917719, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-7574504, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-7660125, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-8559254, http://linkedlifedata.com/resource/pubmed/commentcorrection/20423331-9819378
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1470-8728
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
429
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-41
pubmed:dateRevised
2011-10-14
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
SnoRNA microarray analysis reveals changes in H/ACA and C/D RNA levels caused by dyskerin ablation in mouse liver.
pubmed:affiliation
Division of Hematology, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural