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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-4-27
pubmed:abstractText
At the end of August 2002, clinical symptoms of bluetongue (BT) (fever between 39 degrees C and 41 degrees C, muco-purulent or bloody nasal discharge, oedema of the lips and the intramandibular space, foot lesions including laminitis and coronitis in some cases, diarrhoea and dysentery) were recorded in Pramenka sheep flocks in north-east Bosnia in August 2002. A total of 9 599 serum samples (ovine: 8 967; bovine: 632) from 40 communities of Bosnia and Herzegovina were tested for the presence of anti-bluetongue virus (BTV) antibodies using competitive enzyme-linked immunosorbent assay (c-ELISA) and the standard agar gel immunodiffusion (AGID) test. The c-ELISA revealed BTV-seropositive reactions in 187 (1.94%) samples and the AGID test detected 141 (1.53%) cases. Complete agreement was recorded between the c-ELISA and AGID test results for bovine sera. These results indicate that the ability of c-ELISA to detect anti-bluetongue virus antibodies in ovine sera was superior to that of the AGID. All positive sera were collected from animals in the river areas of Bosnia and Herzegovina.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:issn
1828-1427
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
562-3
pubmed:articleTitle
Bluetongue in Bosnia: comparisons of competitive enzyme-linked immunosorbent assay and standard agar gel immunodiffusion tests.
pubmed:affiliation
Institute of Epidemiology, Veterinary Faculty of the University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina.
pubmed:publicationType
Journal Article