Linked Life Data

20420682

Source:http://linkedlifedata.com/resource/pubmed/id/20420682

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-5-18
pubmed:abstractText
Trigonopsis variabilis D-amino acid oxidase (TvDAO) is a well characterized enzyme used for cephalosporin C conversion on industrial scale. However, the demands on the enzyme with respect to activity, operational stability and costs also vary with the field of application. Processes that use the soluble enzyme suffer from fast inactivation of TvDAO while immobilized oxidase preparations raise issues related to expensive carriers and catalyst efficiency. Therefore, oxidase preparations that are more robust and active than those currently available would enable a much broader range of economically viable applications of this enzyme in fine chemical syntheses. A multi-step engineering approach was chosen here to develop a robust and highly active Pichia pastoris TvDAO whole-cell biocatalyst.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1475-2859
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
24
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst.
pubmed:affiliation
Austrian Centre of Industrial Biotechnology, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't