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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2010-5-20
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pubmed:abstractText |
A peptide nucleic acid (PNA) targeting a splice junction of the murine PTEN primary transcript was covalently conjugated to various basic peptides. When systemically administered to healthy mice, the conjugates displayed sequence-specific alteration of PTEN mRNA splicing as well as inhibition of full length PTEN protein expression. Correlating activity with drug concentration in various tissues indicated strong tissue-dependence, with highest levels of activity observed in adipose tissue. While the presence of a peptide carrier was found to be crucial for efficient delivery to tissue, little difference was observed between the various peptides evaluated. A second PNA-conjugate targeting the murine insulin receptor primary transcript showed a similar activity profile, suggesting that short basic peptides can generally be used to effectively deliver peptide nucleic acids to adipose tissue.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-11574678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-11747141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-11916922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-12074366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-14770178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-14960586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-15148357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-15687490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-15802066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-1602013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-16220989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-16610796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-17233629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-18239599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-1962210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-7692304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-7945427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-8602363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-9128104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-9189140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20420385-998803
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/PTEN Phosphohydrolase,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Nucleic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Pten protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Splice Sites,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Insulin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1520-4804
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pubmed:author |
pubmed-author:AlbertshoferKlausK,
pubmed-author:BennettC FrankCF,
pubmed-author:BerdejaAndresA,
pubmed-author:CoreyDavid RDR,
pubmed-author:GausHansH,
pubmed-author:GriffeyRichard HRH,
pubmed-author:HuJiaxinJ,
pubmed-author:KinbergerGarth AGA,
pubmed-author:MaierMartin AMA,
pubmed-author:MoniaBrett PBP,
pubmed-author:NulfChristopher JCJ,
pubmed-author:SiwkowskiAndrew MAM,
pubmed-author:SwayzeEric EEE,
pubmed-author:VickersTimothy ATA,
pubmed-author:WancewiczEdward VEV,
pubmed-author:WingerTheodore MTM
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pubmed:issnType |
Electronic
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pubmed:day |
27
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3919-26
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:20420385-Adipose Tissue,
pubmed-meshheading:20420385-Animals,
pubmed-meshheading:20420385-Cell Line,
pubmed-meshheading:20420385-Drug Carriers,
pubmed-meshheading:20420385-Kidney,
pubmed-meshheading:20420385-Liver,
pubmed-meshheading:20420385-Male,
pubmed-meshheading:20420385-Mice,
pubmed-meshheading:20420385-Mice, Inbred BALB C,
pubmed-meshheading:20420385-Oligopeptides,
pubmed-meshheading:20420385-PTEN Phosphohydrolase,
pubmed-meshheading:20420385-Peptide Nucleic Acids,
pubmed-meshheading:20420385-RNA, Antisense,
pubmed-meshheading:20420385-RNA, Messenger,
pubmed-meshheading:20420385-RNA Splice Sites,
pubmed-meshheading:20420385-RNA Splicing,
pubmed-meshheading:20420385-Receptor, Insulin,
pubmed-meshheading:20420385-Structure-Activity Relationship,
pubmed-meshheading:20420385-Tissue Distribution
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pubmed:year |
2010
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pubmed:articleTitle |
Peptide nucleic acids conjugated to short basic peptides show improved pharmacokinetics and antisense activity in adipose tissue.
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pubmed:affiliation |
Isis Pharmaceuticals, Inc., Carlsbad, California 92008, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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