20420377

Source:http://linkedlifedata.com/resource/pubmed/id/20420377

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0006935, umls-concept:C0011209, umls-concept:C0013089, umls-concept:C0032521, umls-concept:C0063083, umls-concept:C0178719, umls-concept:C0243144, umls-concept:C0334227, umls-concept:C1527249
pubmed:issue	5
pubmed:dateCreated	2010-5-25
pubmed:abstractText	Understanding the interactions between drug carriers and cells is of importance to enhance the delivery of therapeutics. The release of therapeutics into different intracellular environments, such as the lysosomes or the cell cytoplasm, will impact their pharmacological activity. Herein, we investigate the intracellular fate of layer-by-layer (LbL)-assembled, submicrometer-sized polymer hydrogel capsules in a human colon cancer derived cell line, LIM1899. The cellular uptake of the disulfide-stabilized poly(methacrylic acid) (PMA(SH)) capsules by colon cancer cells is a time-dependent process. Confocal laser scanning microscopy and transmission electron microscopy reveal that the internalized capsules are deformed in membrane-enclosed compartments, which further mature to late endosomes or lysosomes. We further demonstrate the utility of these redox-responsive PMA(SH) capsules for the delivery of doxorubicin (DOX) to colon cancer cells. The DOX-loaded PMA(SH) capsules demonstrate a 5000-fold enhanced cytotoxicity in cell viability studies compared to free DOX.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/101313589
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Disulfides, http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin, http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers, http://linkedlifedata.com/resource/pubmed/chemical/Hydrogels, http://linkedlifedata.com/resource/pubmed/chemical/Polymers, http://linkedlifedata.com/resource/pubmed/chemical/Silicon Dioxide
pubmed:status	MEDLINE
pubmed:month	May
pubmed:issn	1936-086X
pubmed:author	pubmed-author:CarusoFrankF, pubmed-author:DoddsSarah JSJ, pubmed-author:FergusonCharlesC, pubmed-author:HeathJoan KJK, pubmed-author:JohnstonAngus P RAP, pubmed-author:KamphuisMarloes M JMM, pubmed-author:NiceEdouard CEC, pubmed-author:PartonRobert GRG, pubmed-author:XXX
pubmed:issnType	Electronic
pubmed:day	25
pubmed:volume	4
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	2928-36
pubmed:dateRevised	2010-11-18
pubmed:meshHeading	pubmed-meshheading:20420377-Biological Transport, pubmed-meshheading:20420377-Cell Line, Tumor, pubmed-meshheading:20420377-Cell Nucleus, pubmed-meshheading:20420377-Colorectal Neoplasms, pubmed-meshheading:20420377-Diffusion, pubmed-meshheading:20420377-Disulfides, pubmed-meshheading:20420377-Doxorubicin, pubmed-meshheading:20420377-Drug Carriers, pubmed-meshheading:20420377-Endosomes, pubmed-meshheading:20420377-Humans, pubmed-meshheading:20420377-Hydrogels, pubmed-meshheading:20420377-Hydrogen-Ion Concentration, pubmed-meshheading:20420377-Hydrophobic and Hydrophilic Interactions, pubmed-meshheading:20420377-Intracellular Space, pubmed-meshheading:20420377-Lysosomes, pubmed-meshheading:20420377-Particle Size, pubmed-meshheading:20420377-Polymers, pubmed-meshheading:20420377-Silicon Dioxide
pubmed:year	2010
pubmed:articleTitle	Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for Doxorubicin delivery to colorectal cancer cells.
pubmed:affiliation	Department of Chemical and Biomolecular Engineering, The University of Melbourne, Centre for Nanoscience and Nanotechnology, Victoria 3010, Australia.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't