Linked Life Data

20419630

Source:http://linkedlifedata.com/resource/pubmed/id/20419630

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-4-26
pubmed:abstractText
It is envisioned that for the regeneration of highly organized structures, like tendon and ligaments, only aligned fibrous scaffolds can provide adequate topographic guidance to cells. In this study, a novel method to electrospin an aligned scaffold is presented. Electrospun fibres were deposited into a water bath and then the fibres were drawn to a rotating mandrel in a controlled manner. In this way, parallel and cross-aligned fibrous poly (lactide-co-glycolide) (PLGA) scaffolds were fabricated, which were subsequently used to study their effect on the growth behaviour of rat periodontal ligament (PDL) cells. First, the scaffolds were characterized regarding mechanical properties, scaffold stability and degradation in vitro. Then, rat PDL cells were seeded and cultured on these scaffolds for up to 7 days. Randomly oriented PLGA and solvent cast plain PLGA films served as controls. Results showed that the alignment of fibres resulted in a higher tensile stress and Young's modulus. Aligned scaffolds maintained their structural stability better compared to the controls after incubation in phosphate-buffered saline for 6 weeks. Further, cells were observed to elongate along the fibre after 3 days of culture. Proliferation and migration of PDL cells was significantly more prevalent on the aligned fibres compared to the controls. It was concluded that aligned scaffolds seem to be able to promote the organized regeneration of periodontal tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1473-2262
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
180-92
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
The effect of electrospun fibre alignment on the behaviour of rat periodontal ligament cells.
pubmed:affiliation
Department of Biomaterials, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't