Source:http://linkedlifedata.com/resource/pubmed/id/20417202
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2010-8-3
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| pubmed:abstractText |
Leishmania major (L. major) signal peptidase type I (SPase I) is an endopeptidase encoded by a single-copy gene. In all organisms, SPase I is responsible for removing the signal peptide from secretory pre-proteins and releasing mature proteins to cellular or extra-cellular space. In this study, the role of SPase I in L. major is investigated by gene deletion using homologous recombination (HR). The null mutant of SPase I was not possible to create, suggesting that SPase I is an essential gene for parasite survival. The obtained heterozygote mutant by disrupting one allele of SPase I in L. major showed significantly reduced level of infectivity in bone marrow-derived macrophages. In addition, the heterozygote mutants are unable to cause cutaneous lesion in susceptible BALB/c mice. This is the first report showing that SPase I may have an important role in Leishmania infectivity, e.g. in differentiation and survival of amastigotes. Apparently, the SPase I expression is not essential for in vitro growth of the parasite.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1090-2449
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2010 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
126
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
135-45
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| pubmed:meshHeading |
pubmed-meshheading:20417202-Animals,
pubmed-meshheading:20417202-Blotting, Western,
pubmed-meshheading:20417202-Cells, Cultured,
pubmed-meshheading:20417202-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:20417202-Genetic Vectors,
pubmed-meshheading:20417202-Genotype,
pubmed-meshheading:20417202-Leishmania major,
pubmed-meshheading:20417202-Leishmaniasis, Cutaneous,
pubmed-meshheading:20417202-Macrophages,
pubmed-meshheading:20417202-Membrane Proteins,
pubmed-meshheading:20417202-Mice,
pubmed-meshheading:20417202-Mice, Inbred BALB C,
pubmed-meshheading:20417202-Mutation,
pubmed-meshheading:20417202-Plasmids,
pubmed-meshheading:20417202-RNA, Messenger,
pubmed-meshheading:20417202-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20417202-Serine Endopeptidases,
pubmed-meshheading:20417202-Transfection
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Leishmania major: disruption of signal peptidase type I and its consequences on survival, growth and infectivity.
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| pubmed:affiliation |
Molecular Immunology and Vaccine Research Lab, Pasteur Institute of Iran, Tehran, Iran.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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