Linked Life Data

20416388

Source:http://linkedlifedata.com/resource/pubmed/id/20416388

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-7-12
pubmed:abstractText
Hydrogen sulfide (H(2)S) inhibits the last step of the denitrification process, i.e. the reduction of nitrous oxide (N(2)O) to dinitrogen gas (N(2)), both in natural environments (marine sediments) and industrial processes (activated sludge, methanogenic sludge, BioDeNOx process). In a previously published study, we showed that the inhibitory effect of sulfide to N(2)O reduction in mixed microbial communities is reversible and can be counteracted by dosing trace amounts of copper. It remained, however, unclear if this was due to copper sulfide precipitation or a retrofitting of the copper containing N(2)O-reductase (N(2)OR). The present study aimed to elucidate the mechanism of the restoration of sulfide-inhibited N(2)O reducing activity by metal addition to a pure Pseudomonas aeruginosa culture. This was done by using other metals (zinc, cobalt and iron) in comparison with copper. Zinc and cobalt clearly alleviated the sulfide inhibition of N(2)OR to the same extent as copper and the activity restoration was extremely fast (within 15 min, Fig. 3) for zinc, cobalt and copper. This suggests that the alleviation of the inhibitory effect of sulfide is due to metal sulfide precipitation and thus not exclusively limited to Cu. This work also underlines the importance of metal speciation: supply of iron did not restore the N(2)OR activity because it was precipitated by the phosphates present in the medium and thus could not precipitate the sulfide.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1089-8611
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-5
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Divalent metal addition restores sulfide-inhibited N(2)O reduction in Pseudomonas aeruginosa.
pubmed:affiliation
Sub-department of Environmental Technology, Wageningen University, Biotechnion-Bomenweg 2, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. j.bartacek@unesco-ihe.org
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't