Source:http://linkedlifedata.com/resource/pubmed/id/20416132
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2010-7-21
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pubmed:abstractText |
Quercetin has been described as having a wide range of beneficial effects in humans, ranging from anti-carcinogenic properties to reducing the risk of CVD. Nevertheless, underlying molecular mechanisms have been mostly investigated in vitro. Here, we tested whether a daily supplementation of quercetin leads to reproducible changes in human monocyte gene expression profiles. In study I, quercetin in varying dosages was given to healthy subjects for 2 weeks. RNA from monocytes isolated at the beginning and end of the study from subjects receiving 150 mg quercetin per d was subjected to transcriptome-wide microarray analysis. In study II, a double-blind cross-over study, twenty subjects exhibiting a 'cardiovascular risk phenotype' received 150 mg quercetin or placebo daily for 6 weeks each and served as the verification group. Microarray analysis revealed a number of differentially expressed genes. The most significantly represented functional groups were those of the immune system, nucleic acid metabolism, apoptosis and O-glycan biosynthesis. Twenty-four genes were chosen for technical replication and independent verification by quantitative real-time PCR. When comparing placebo and quercetin treatment, four genes showed significantly different expression changes (C1GALT1, O-glycan biosynthesis; GM2A, glycolipid catabolism; HDGF, cell proliferation; SERPINB9, apoptosis). However, these were minimal in respect to magnitude of fold change. In conclusion, although microarray analysis revealed extensive effects of quercetin on gene expression, the employment of a placebo-controlled study design showed no comparable results for twenty-four verification targets. This emphasises the need for stringent designs in nutritional intervention studies with the aim to identify relevant changes in gene expression.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Quercetin
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1475-2662
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
336-45
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pubmed:meshHeading |
pubmed-meshheading:20416132-Adult,
pubmed-meshheading:20416132-Antioxidants,
pubmed-meshheading:20416132-Apoptosis,
pubmed-meshheading:20416132-Cardiovascular Diseases,
pubmed-meshheading:20416132-Cross-Over Studies,
pubmed-meshheading:20416132-Dietary Supplements,
pubmed-meshheading:20416132-Double-Blind Method,
pubmed-meshheading:20416132-Female,
pubmed-meshheading:20416132-Gene Expression,
pubmed-meshheading:20416132-Gene Expression Profiling,
pubmed-meshheading:20416132-Humans,
pubmed-meshheading:20416132-Immune System,
pubmed-meshheading:20416132-Male,
pubmed-meshheading:20416132-Middle Aged,
pubmed-meshheading:20416132-Monocytes,
pubmed-meshheading:20416132-Nucleic Acids,
pubmed-meshheading:20416132-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:20416132-Phenotype,
pubmed-meshheading:20416132-Plant Extracts,
pubmed-meshheading:20416132-Polysaccharides,
pubmed-meshheading:20416132-Quercetin,
pubmed-meshheading:20416132-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20416132-Risk,
pubmed-meshheading:20416132-Young Adult
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pubmed:year |
2010
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pubmed:articleTitle |
Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo.
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pubmed:affiliation |
Department of Molecular Prevention, Institute of Human Nutrition and Food Science, Christian-Albrechts University Kiel, Kiel, Germany.
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pubmed:publicationType |
Journal Article,
Randomized Controlled Trial,
Research Support, Non-U.S. Gov't
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