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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-7-21
pubmed:abstractText
Quercetin has been described as having a wide range of beneficial effects in humans, ranging from anti-carcinogenic properties to reducing the risk of CVD. Nevertheless, underlying molecular mechanisms have been mostly investigated in vitro. Here, we tested whether a daily supplementation of quercetin leads to reproducible changes in human monocyte gene expression profiles. In study I, quercetin in varying dosages was given to healthy subjects for 2 weeks. RNA from monocytes isolated at the beginning and end of the study from subjects receiving 150 mg quercetin per d was subjected to transcriptome-wide microarray analysis. In study II, a double-blind cross-over study, twenty subjects exhibiting a 'cardiovascular risk phenotype' received 150 mg quercetin or placebo daily for 6 weeks each and served as the verification group. Microarray analysis revealed a number of differentially expressed genes. The most significantly represented functional groups were those of the immune system, nucleic acid metabolism, apoptosis and O-glycan biosynthesis. Twenty-four genes were chosen for technical replication and independent verification by quantitative real-time PCR. When comparing placebo and quercetin treatment, four genes showed significantly different expression changes (C1GALT1, O-glycan biosynthesis; GM2A, glycolipid catabolism; HDGF, cell proliferation; SERPINB9, apoptosis). However, these were minimal in respect to magnitude of fold change. In conclusion, although microarray analysis revealed extensive effects of quercetin on gene expression, the employment of a placebo-controlled study design showed no comparable results for twenty-four verification targets. This emphasises the need for stringent designs in nutritional intervention studies with the aim to identify relevant changes in gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1475-2662
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
336-45
pubmed:meshHeading
pubmed-meshheading:20416132-Adult, pubmed-meshheading:20416132-Antioxidants, pubmed-meshheading:20416132-Apoptosis, pubmed-meshheading:20416132-Cardiovascular Diseases, pubmed-meshheading:20416132-Cross-Over Studies, pubmed-meshheading:20416132-Dietary Supplements, pubmed-meshheading:20416132-Double-Blind Method, pubmed-meshheading:20416132-Female, pubmed-meshheading:20416132-Gene Expression, pubmed-meshheading:20416132-Gene Expression Profiling, pubmed-meshheading:20416132-Humans, pubmed-meshheading:20416132-Immune System, pubmed-meshheading:20416132-Male, pubmed-meshheading:20416132-Middle Aged, pubmed-meshheading:20416132-Monocytes, pubmed-meshheading:20416132-Nucleic Acids, pubmed-meshheading:20416132-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:20416132-Phenotype, pubmed-meshheading:20416132-Plant Extracts, pubmed-meshheading:20416132-Polysaccharides, pubmed-meshheading:20416132-Quercetin, pubmed-meshheading:20416132-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20416132-Risk, pubmed-meshheading:20416132-Young Adult
pubmed:year
2010
pubmed:articleTitle
Quercetin supplementation and its effect on human monocyte gene expression profiles in vivo.
pubmed:affiliation
Department of Molecular Prevention, Institute of Human Nutrition and Food Science, Christian-Albrechts University Kiel, Kiel, Germany.
pubmed:publicationType
Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't