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rdf:type |
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lifeskim:mentions |
umls-concept:C0001347,
umls-concept:C0014597,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0205225,
umls-concept:C0227525,
umls-concept:C0282547,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1882726,
umls-concept:C2349975,
umls-concept:C2911692
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pubmed:issue |
3
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pubmed:dateCreated |
2010-6-9
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pubmed:abstractText |
Three different primary rat hepatocyte culture methods were compared for their ability to allow the secretion of fibrinogen and albumin under basal and IL-6-stimulated conditions. These culture methods comprised the co-culture of hepatocytes with rat liver epithelial cells (CC-RLEC), a collagen type I sandwich culture (SW) and a conventional primary hepatocyte monolayer culture (ML). Basal albumin secretion was most stable over time in SW. Fibrinogen secretion was induced by IL-6 in all cell culture models. Compared with ML, CC-RLEC showed an almost three-fold higher fibrinogen secretion under both control and IL-6-stimulated conditions. Induction of fibrinogen release by IL-6 was lowest in SW. Albumin secretion was decreased after IL-6 stimulation in both ML and CC-RLEC. Thus, cells growing under the various primary hepatocyte cell culture techniques react differently to IL-6 stimulation with regard to acute-phase protein secretion. CC-RLEC is the preferred method for studying cytokine-mediated induction of acute-phase proteins, because of the pronounced stimulation of fibrinogen secretion upon IL-6 exposure under these conditions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-10334936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-10633294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-10633295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-10681645,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-14991941,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-1689567,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-1707930,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-7778689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-8236274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-8238878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-8342601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-9155709,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20411395-9688980
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1432-0878
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
340
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
451-7
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pubmed:dateRevised |
2010-9-30
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pubmed:meshHeading |
pubmed-meshheading:20411395-Acute-Phase Proteins,
pubmed-meshheading:20411395-Acute-Phase Reaction,
pubmed-meshheading:20411395-Albumins,
pubmed-meshheading:20411395-Animals,
pubmed-meshheading:20411395-Cell Shape,
pubmed-meshheading:20411395-Cells, Cultured,
pubmed-meshheading:20411395-Coculture Techniques,
pubmed-meshheading:20411395-Epithelial Cells,
pubmed-meshheading:20411395-Fibrinogen,
pubmed-meshheading:20411395-Hepatocytes,
pubmed-meshheading:20411395-Interleukin-6,
pubmed-meshheading:20411395-Liver,
pubmed-meshheading:20411395-Male,
pubmed-meshheading:20411395-Rats,
pubmed-meshheading:20411395-Rats, Sprague-Dawley,
pubmed-meshheading:20411395-Reproducibility of Results
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pubmed:year |
2010
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pubmed:articleTitle |
Co-culture of primary rat hepatocytes with rat liver epithelial cells enhances interleukin-6-induced acute-phase protein response.
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