Linked Life Data

20408670

Source:http://linkedlifedata.com/resource/pubmed/id/20408670

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-4-22
pubmed:abstractText
Solid-supported bilayer lipid membranes (SBLMs) containing membrane protein have been generated through a simple lipid dilution technique. SBLM formation from mixtures of native Escherichia coli bacterial inner membrane (IM) vesicles diluted with egg phosphatidylcholine (egg PC) vesicles has been explored with dissipation enhanced quartz crystal microbalance (QCM-D), atomic force microscopy (AFM), attenuated total internal-reflection Fourier-transform infrared spectroscopy (ATR-FTIR), and fluorescence recovery after photobleaching (FRAP). QCM-D studies reveal that SBLM formation from vesicle mixtures ranging between 0% and 100% IM can be divided into two regimes. Samples with < or = 40% IM form SBLMs, while samples of greater IM fractions are dominated by vesicle adsorption. FRAP experiments showed that the bilayers formed from mixed vesicles with < or = 40% IM were fluid, and comprised a mixture of both egg PC and IM. ATR-FTIR measurements on SBLMs membranes formed with 30% IM confirm that protein is present. SBLM formation was also explored as a function of temperature by QCM-D and FRAP. For samples of 30% IM, QCM-D data show a decreased mass and viscoelasticity at elevated temperatures, and an increased fluidity is observed by FRAP measurements. These results suggest improved biomimetic characteristics can be obtained by forming and maintaining the system at, or close to, 37 degrees C.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jun
pubmed:issn
1559-4106
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
FA59
pubmed:year
2008
pubmed:articleTitle
Native E. coli inner membrane incorporation in solid-supported lipid bilayer membranes.
pubmed:affiliation
School of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't