Linked Life Data

20408627

Source:http://linkedlifedata.com/resource/pubmed/id/20408627

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-4-22
pubmed:abstractText
Membrane protein microarrays are expected to play a key role in the future of drug screening and discovery. The authors present a method for the creation of functional heterogeneous vesicle arrays via DNA controlled surface sorting. Complexes of streptavidin and biotinylated DNA are spotted onto a biomolecule- and cell-resistant surface of biotinylated poly(L-lysine)-grafted-poly(ethylene glycol). Two kinds of vesicles functionalized with either the membrane-binding protein annexin A5 or loaded with bovine serum albumin, are tagged with DNA, mixed together, and guided to predefined spots on the surface. The authors show that the spotted complexes remain active and selective and that the background is resistant towards nonspecific adsorption of the vesicles and the proteins.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Dec
pubmed:issn
1559-4106
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
142-5
pubmed:year
2006
pubmed:articleTitle
Creation of a functional heterogeneous vesicle array via DNA controlled surface sorting onto a spotted microarray.
pubmed:affiliation
Laboratory of Biosensors and Bioelectronics, Institute for Medical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, 8092 Zurich, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't