Linked Life Data

20408521

Source:http://linkedlifedata.com/resource/pubmed/id/20408521

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2010-5-5
pubmed:abstractText
A method is presented for screening high-density arrays to discover peptides that bind and modulate enzyme activity. A polyvinyl alcohol solution was applied to array surfaces to limit the diffusion of product molecules released from enzymatic reactions, allowing the simultaneous measurement of enzyme activity and binding at each peptide spot. For proof of concept, it was possible to identify peptides that bound to horseradish peroxidase, alkaline phosphatase, and beta-galactosidase and substantially altered enzyme activity by comparing the binding level of peptide to enzyme and bound enzyme activity. This basic technique may be generally applicable to find peptides or other small molecules that modify enzyme activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1520-5126
pubmed:author
pubmed:issnType
Electronic
pubmed:day
12
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6419-24
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Exploring peptide space for enzyme modulators.
pubmed:affiliation
Center for Single Molecule Biophysics, Arizona State University, Tempe, Arizona 85287, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't