Linked Life Data

20407940

Source:http://linkedlifedata.com/resource/pubmed/id/20407940

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-4-21
pubmed:abstractText
Urine is a complex fluid, which is thought to contain valuable diagnostic information regarding general health. In particular, there is great diagnostic potential in the peptide and/or protein content of urine, but the information is present in low abundance. Most traditional proteomic techniques lack sufficient sensitivity/dynamic range, especially for dilute and/or complex samples. However, orthogonal separation methods can be applied prior to protein/peptide analysis to increase the success rate of urine proteomic studies and access this potentially valuable information. In this chapter, we describe isoelectric focusing (IEF) of intact urine proteins, via free flow electrophoresis (FFE), prior to typical peptide-based mass spectrometry analysis, facilitating the deep analysis of urine protein detection and identification, for biomarker discovery. Our work demonstrates that such an approach can be used as a preprocessing step and can be integrated into a workflow for the successful identification of protein components (biomarkers) from urine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
641
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-45
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Application of free flow electrophoresis to the analysis of the urine proteome.
pubmed:affiliation
BD Diagnostic, Franklin Lakes, NJ, USA.
pubmed:publicationType
Journal Article