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rdf:type |
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lifeskim:mentions |
umls-concept:C0013935,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0040661,
umls-concept:C0086597,
umls-concept:C0164786,
umls-concept:C0166894,
umls-concept:C0441655,
umls-concept:C0814002,
umls-concept:C1113654,
umls-concept:C1367307,
umls-concept:C1705162,
umls-concept:C1709059,
umls-concept:C1710082
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pubmed:issue |
6
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pubmed:dateCreated |
2010-8-2
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pubmed:abstractText |
The effect of neurotrophin-4 (Ntf4) on mouse embryonic (day-14) neural stem cell (mE14-NSC) fate determination and the mechanisms involved were investigated. Using primary mE14-NSCs, immunocytochemistry and molecular-cell biological methods, such as Western-blotting, we characterized the effect of Ntf4 on mE14-NSC differentiation. Obtained in-vitro data revealed an interesting phenomenon of Ntf4 action resulting in enhanced mE14-NSC commitment to progenitor cells of the neuronal lineage. During this process, Ntf4 suppresses the interleukin 6 (Il6) family receptor and the Notch signalling pathways by modulating their specific receptor cleavages. The observed lineage commitment is controlled via an Ntf4-mediated modulation of protein kinase B (PKB/Akt) activity and characterized by a decreased Stat3 (signal transducer and activator of transcription-3) phosphorylation status. These findings suggest that the Ntf4-activated signalling cascade is responsible for initiating a concert among sheddases, kinases, and phosphatases to mediate neurogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1573-6830
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
909-16
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pubmed:meshHeading |
pubmed-meshheading:20407817-Animals,
pubmed-meshheading:20407817-Cell Differentiation,
pubmed-meshheading:20407817-Embryonic Stem Cells,
pubmed-meshheading:20407817-Mice,
pubmed-meshheading:20407817-Mice, Inbred C57BL,
pubmed-meshheading:20407817-Models, Biological,
pubmed-meshheading:20407817-Nerve Growth Factors,
pubmed-meshheading:20407817-Neural Stem Cells,
pubmed-meshheading:20407817-Neurogenesis,
pubmed-meshheading:20407817-Neurons,
pubmed-meshheading:20407817-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:20407817-STAT3 Transcription Factor,
pubmed-meshheading:20407817-Signal Transduction,
pubmed-meshheading:20407817-Stem Cell Factor,
pubmed-meshheading:20407817-Time Factors
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pubmed:year |
2010
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pubmed:articleTitle |
Neurotrophin-4 (ntf4) mediates neurogenesis in mouse embryonic neural stem cells through the inhibition of the signal transducer and activator of transcription-3 (stat3) and the modulation of the activity of protein kinase B.
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pubmed:affiliation |
Department of Molecular and Cell Biology, School of Biological Sciences, College of Science, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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