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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1991-7-10
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pubmed:abstractText |
Activating the protein-tyrosine kinase activity of v-Src in murine fibroblasts leads to increased expression of Egr-1, a mitogen-responsive transcription factor. v-Src-induced expression of Egr-1 is independent of protein synthesis and is controlled at the level of transcription. Target sequences responsive to v-Src-induced signals were investigated using deletion mutant and analysis of the Egr-1 promoter. Upstream Egr-1 promoter sequences linked to a reporter gene were cotransfected with a v-Src expression vector into NIH 3T3 cells. v-Src-enhanced gene expression from the Egr-1 promoter was dependent upon the presence of CC(A/T)6GG elements. The CC(A/T)6GG motif forms the core element of serum response elements (SREs) and is the binding site for serum response factor. The Egr-1 promoter sequences responsive to v-Src contained four SREs. Sequential deletion of these SREs reduced v-Src responsiveness to basal transcription levels. A single SRE from this region was able to confer v-Src responsiveness to a heterologous promoter, and a mutation to the CC(A/T)6GG box of this SRE abolished v-Src-enhanced gene expression. Thus, an early response of v-Src-induced intracellular signaling is the transcriptional activation of a growth factor-responsive transcription factor via an SRE.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Egr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein pp60(v-src),
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Response Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
266
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10802-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2040600-Animals,
pubmed-meshheading:2040600-Base Sequence,
pubmed-meshheading:2040600-Cell Line,
pubmed-meshheading:2040600-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:2040600-Chromosome Deletion,
pubmed-meshheading:2040600-DNA-Binding Proteins,
pubmed-meshheading:2040600-Early Growth Response Protein 1,
pubmed-meshheading:2040600-Gene Expression Regulation,
pubmed-meshheading:2040600-Immediate-Early Proteins,
pubmed-meshheading:2040600-Mice,
pubmed-meshheading:2040600-Mice, Inbred BALB C,
pubmed-meshheading:2040600-Molecular Sequence Data,
pubmed-meshheading:2040600-Nuclear Proteins,
pubmed-meshheading:2040600-Oligonucleotide Probes,
pubmed-meshheading:2040600-Oncogene Protein pp60(v-src),
pubmed-meshheading:2040600-Plasmids,
pubmed-meshheading:2040600-Promoter Regions, Genetic,
pubmed-meshheading:2040600-Protein-Tyrosine Kinases,
pubmed-meshheading:2040600-RNA, Messenger,
pubmed-meshheading:2040600-Serum Response Factor,
pubmed-meshheading:2040600-Transcription, Genetic,
pubmed-meshheading:2040600-Transcription Factors,
pubmed-meshheading:2040600-Transfection,
pubmed-meshheading:2040600-Zinc Fingers
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pubmed:year |
1991
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pubmed:articleTitle |
v-Src activates mitogen-responsive transcription factor Egr-1 via serum response elements.
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pubmed:affiliation |
Institute for Biomolecular Structure and Function, Hunter College City University of New York, New York 10021.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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