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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-7-10
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pubmed:abstractText |
Faecal alpha 1 antitrypsin was determined in 34 patients with Crohn's disease and in 19 healthy subjects by immune nephelometry. A structural analysis of faecal alpha 1 antitrypsin was carried out using immunoblot analysis under non-reducing conditions. Native serum alpha 1 antitrypsin migrated with an apparent molecular weight of 45 kDa. Proteolytic alpha 1 antitrypsin fragments (5-42 kDa) were specifically immunostained in 13/19 and 22/34 stool samples from control subjects and from patients with Crohn's disease respectively. There was a weak correlation (r = 0.47; p less than 0.02) between the molecular weight of fragmented alpha 1 antitrypsin and the faecal concentration in both groups, indicating that alpha 1 antitrypsin inhibits its own proteolysis by intestinal proteases in a dose dependent way. The incidence of polymeric forms (greater than 45 kDa) was similar in patients (10/34) and control subjects (5/19). In only one case in each group was the native serum form of alpha 1 antitrypsin found in faeces. We conclude that faecal alpha 1 antitrypsin differs structurally from the native serum form. Immunochemical measurements, therefore, reflect rather than represent faecal concentrations of alpha 1 antitrypsin. The controversial results in published reports may be partly explained by these findings. The molecular heterogeneity of faecal alpha 1 antitrypsin is not specifically associated with Crohn's disease.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-1582608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-2784776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-2806991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-300037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-3257935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-3263222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-3263408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-3489090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-3495470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-3874110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-3919562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-6603392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-6970702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-80688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2040471-91891
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0017-5749
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
496-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2040471-Adult,
pubmed-meshheading:2040471-Crohn Disease,
pubmed-meshheading:2040471-Feces,
pubmed-meshheading:2040471-Female,
pubmed-meshheading:2040471-Humans,
pubmed-meshheading:2040471-Immunoblotting,
pubmed-meshheading:2040471-Male,
pubmed-meshheading:2040471-Middle Aged,
pubmed-meshheading:2040471-Molecular Weight,
pubmed-meshheading:2040471-Nephelometry and Turbidimetry,
pubmed-meshheading:2040471-Protein Conformation,
pubmed-meshheading:2040471-alpha 1-Antitrypsin
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pubmed:year |
1991
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pubmed:articleTitle |
Structural heterogeneity of faecal alpha 1 antitrypsin shown by immunoblot analysis in patients with Crohn's disease.
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pubmed:affiliation |
Medizinische Poliklinik, University of Würzburg, FRG.
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pubmed:publicationType |
Journal Article
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