Linked Life Data

20403458

Source:http://linkedlifedata.com/resource/pubmed/id/20403458

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2010-7-19
pubmed:abstractText
Fish are the primary source in the human food basket of the n-3 long-chain polyunsaturated fatty acids, eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), that are crucial to the health of higher vertebrates. Atlantic salmon are able to synthesize EPA and DHA from 18:3n-3 through reactions catalyzed by fatty acyl desaturases (Fad) and elongases of very long chain fatty acids. Previously, two cDNAs encoding functionally distinct Delta5 and Delta6 Fads were isolated, but screening of a genomic DNA library revealed the existence of more putative fad genes in the Atlantic salmon genome. In the present study, we show that there are at least four genes encoding putative Fad proteins in Atlantic salmon. Two genes, Delta6fad_a and Delta5fad, corresponded to the previously cloned Delta6 and Delta5 Fad cDNAs. Functional characterization by heterologous expression in yeast showed that the cDNAs for both the two further putative fad genes, Delta6fad_b and Delta6fad_c, had only Delta6 activity, converting 47 % and 12 % of 18:3n-3 to 18:4n-3, and 25 and 7 % of 18:2n-6 to 18:3n-6, for 6Fad_b and Delta6fad_c, respectively. Both 6fad_a and 6fad_b genes were highly expressed in intestine (pyloric caeca), liver and brain, with 6fad_b also highly expressed in gill, whereas 6fad_c transcript was found predominantly in brain, with lower expression levels in all other tissues. The expression levels of the 6fad_a gene in liver and the 6fad_b gene in intestine were significantly higher in fish fed diets containing vegetable oil compared to fish fed fish oil suggesting up-regulation in response to reduced dietary EPA and DHA. In contrast, no significant differences were found between transcript levels for 6fad_a in intestine, 6fad_b in liver, or 6fad_c in liver or intestine of fish fed vegetable oil compared to fish fed fish oil. The observed differences in tissue expression and nutritional regulation of the fad genes are discussed in relation to gene structures and fish physiology.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-3002
pubmed:author
pubmed:copyrightInfo
2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Print
pubmed:volume
1801
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1072-81
pubmed:meshHeading
pubmed-meshheading:20403458-Amino Acid Sequence, pubmed-meshheading:20403458-Animals, pubmed-meshheading:20403458-Cloning, Molecular, pubmed-meshheading:20403458-DNA, Complementary, pubmed-meshheading:20403458-Docosahexaenoic Acids, pubmed-meshheading:20403458-Eicosapentaenoic Acid, pubmed-meshheading:20403458-Fatty Acid Desaturases, pubmed-meshheading:20403458-Fish Oils, pubmed-meshheading:20403458-Genetic Complementation Test, pubmed-meshheading:20403458-Humans, pubmed-meshheading:20403458-Linoleoyl-CoA Desaturase, pubmed-meshheading:20403458-Molecular Sequence Data, pubmed-meshheading:20403458-Open Reading Frames, pubmed-meshheading:20403458-Phylogeny, pubmed-meshheading:20403458-Plant Oils, pubmed-meshheading:20403458-Saccharomyces cerevisiae, pubmed-meshheading:20403458-Salmo salar, pubmed-meshheading:20403458-Sequence Homology, Amino Acid, pubmed-meshheading:20403458-Tissue Distribution
pubmed:year
2010
pubmed:articleTitle
Multiple genes for functional 6 fatty acyl desaturases (Fad) in Atlantic salmon (Salmo salar L.): gene and cDNA characterization, functional expression, tissue distribution and nutritional regulation.
pubmed:affiliation
Institute of Aquaculture, University of Stirling, Stirling FK94LA, Scotland, UK. oscar.monroig@stir.ac.uk
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't