Linked Life Data

20399768

Source:http://linkedlifedata.com/resource/pubmed/id/20399768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2010-5-31
pubmed:abstractText
The nitric oxide (NO)-independent soluble guanylyl cyclase stimulator stimulator BAY 41-2272 was reported to produce relaxant response in different types of smooth muscle. However no study was carried out to investigate the effects of BAY 412282 in detrusor smooth muscle. Thus, this study aimed to evaluate the relaxant effects of BAY 41-2272, in isolated mouse, rat and rabbit detrusor smooth muscle. Mouse, rat and rabbit were anesthetized, and urinary bladder removed. Detrusor smooth muscle was transferred to 10-mL organ baths containing oxygenated and warmed Krebs-Henseleit solution. Tissues were connected to force-displacement transducers and changes in isometric force were recorded. BAY 41-2272 (0.001-100 microM) produced concentration-dependent detrusor smooth muscle relaxations in mouse, rat and rabbit with maximal responses of 61.3+/-6.6%, 95.1+/-9.9% and 91.7+/-5.9%, respectively. Sodium nitroprusside and glyceryl trinitrate, as well as 8-bromo-cGMP also produced detrusor relaxations, but to a much lesser extent than BAY 41-2272. The NO synthesis inhibitor L-NAME and the phosphodiesterase-5 inhibitor sildenafil had no effect in BAY 41-2272-induced responses. However, the soluble guanylyl cyclase inhibitor ODQ significantly reduced BAY 41-2272-induced relaxations. BAY 41-2272 increased the bladder cGMP levels by about of 14- and 20-fold for 10 and 100 microM, respectively, which were markedly reduced by ODQ. The cAMP levels were unaffected by BAY 41-2272. Moreover, BAY 41-2272 significantly reduced the contractile responses to extracellular Ca(2+) in an ODQ-insensitive manner. In conclusion, rabbit detrusor smooth muscle relaxations by BAY 41-2272 involve mainly cGMP production, but an additional mechanism involving Ca(2+) influx blockade independently of cGMP production appears to be involved.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1879-0712
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
10
pubmed:volume
637
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-7
pubmed:meshHeading
pubmed-meshheading:20399768-Animals, pubmed-meshheading:20399768-Calcium Channels, pubmed-meshheading:20399768-Cyclic GMP, pubmed-meshheading:20399768-Dose-Response Relationship, Drug, pubmed-meshheading:20399768-Enzyme Activation, pubmed-meshheading:20399768-Enzyme Activators, pubmed-meshheading:20399768-Guanylate Cyclase, pubmed-meshheading:20399768-Male, pubmed-meshheading:20399768-Mice, pubmed-meshheading:20399768-Mice, Inbred C57BL, pubmed-meshheading:20399768-Muscle, Smooth, pubmed-meshheading:20399768-Muscle Relaxation, pubmed-meshheading:20399768-Nitric Oxide, pubmed-meshheading:20399768-Potassium Channels, pubmed-meshheading:20399768-Pyrazoles, pubmed-meshheading:20399768-Pyridines, pubmed-meshheading:20399768-Rabbits, pubmed-meshheading:20399768-Rats, pubmed-meshheading:20399768-Rats, Wistar, pubmed-meshheading:20399768-Receptors, Cytoplasmic and Nuclear
pubmed:year
2010
pubmed:articleTitle
Evaluation of the relaxant effect of the nitric oxide-independent soluble guanylyl cyclase stimulator BAY 41-2272 in isolated detrusor smooth muscle.
pubmed:affiliation
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas (SP), Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't