Source:http://linkedlifedata.com/resource/pubmed/id/20399441
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
22
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| pubmed:dateCreated |
2010-5-5
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| pubmed:abstractText |
For the first time, an automatic sample pre-treatment/detection method is proposed for the multiclass determination of UV filters (namely, benzophenone-3, ethylhexylmetoxycinnamate, butylmethoxydibenzoylmethane and homosalate) in environmental samples. The new methodology comprises in-line solid-phase extraction (SPE) of the target analytes by exploiting the bead injection (BI) concept in a mesofluidic lab-on-valve (LOV) format, with subsequent determination by liquid chromatography (LC). The proposed microanalytical system, using a multisyringe burette as propulsion unit, automatically performed the overall SPE steps, including the renewal of the sorbent in each analytical cycle to prevent sample cross-contamination and the post-extraction adjustment of the eluate composition to prevent chromatographic band broadening effects. In order to expedite the LC separation, a C(18) monolithic column was applied and an accelerated isocratic elution was carried out by using a cationic surfactant as mobile phase additive. The LOV-BI-LC method was proven reliable for handling and analysis of complex matrices, e.g., spiked swimming pool water and seawater, with limits of detection ranging between 0.45 and 3.2 microg L(-1) for 9 mL sample volume. Linear calibration was attained up to 160 microg L(-1) for homosalate and up to 35 microg L(-1) for the other target analytes, with good reproducibility (RSD<13%, for 5 different SPE columns). The hyphenated scheme is able to process a given sample simultaneously and within the same time frame than the chromatographic separation/determination of the formerly pre-treated sample, providing concentration values every 9 min. Hence, the sample throughput was enhanced up to 33 times when compared with previously reported off-line SPE methods. A drastic reduction in reagent consumption and effluent production was also attained, contributing to the development of an environment-friendly analyzer.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1873-3778
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2010 Elsevier B.V. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
28
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| pubmed:volume |
1217
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
3575-82
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| pubmed:meshHeading |
pubmed-meshheading:20399441-Chlorine,
pubmed-meshheading:20399441-Chromatography, Liquid,
pubmed-meshheading:20399441-Equipment Design,
pubmed-meshheading:20399441-Linear Models,
pubmed-meshheading:20399441-Reproducibility of Results,
pubmed-meshheading:20399441-Seawater,
pubmed-meshheading:20399441-Sensitivity and Specificity,
pubmed-meshheading:20399441-Solid Phase Extraction,
pubmed-meshheading:20399441-Sunscreening Agents,
pubmed-meshheading:20399441-Swimming Pools
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| pubmed:year |
2010
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| pubmed:articleTitle |
On-line renewable solid-phase extraction hyphenated to liquid chromatography for the determination of UV filters using bead injection and multisyringe-lab-on-valve approach.
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| pubmed:affiliation |
REQUIMTE, Serviço de Química-Física, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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