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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0205370,
umls-concept:C0243144,
umls-concept:C0392756,
umls-concept:C0596383,
umls-concept:C0599894,
umls-concept:C1167622,
umls-concept:C1272745,
umls-concept:C1450054,
umls-concept:C1522408,
umls-concept:C1697272,
umls-concept:C1704806,
umls-concept:C2935744
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pubmed:issue |
20
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pubmed:dateCreated |
2010-5-10
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pubmed:abstractText |
One of the major limitations impeding the sensitivity and specificity of biomarker targeted nanoparticles is non-specific binding by biomolecules and uptake by the reticuloendothelial system (RES). We report the development of an antibiofouling polysiloxane containing amphiphilic diblock copolymer, poly(ethylene oxide)-block-poly(gamma-methacryloxypropyl trimethoxysilane) (PEO-b-PgammaMPS), for coating and functionalizing high quality hydrophobic nanocrystals such as iron oxide nanoparticles and quantum dots. These PEO-b-PgammaMPS-coated nanocrystals were colloidally stable in biological medium and showed low non-specific binding by macromolecules after incubation with 100% fetal bovine serum. Both in vitro experiments with macrophages and in vivo biodistribution studies in mice revealed that PEO-b-PgammaMPS copolymer-coated nanocrystals have an antibiofouling effect that reduces non-specific cell and RES uptake. Surface functionalization with amine groups was accomplished through co-crosslinking the polysiloxane coating layer and (3-Aminopropyl)trimethoxysilane in aqueous solution. Tumor integrin alpha(v)beta(3) targeting peptide cyclo-RGD ligands were conjugated on the nanoparticles through a heterobifunctional linker. The resulting integrin alpha(v)beta(3) targeting nanoparticle conjugates showed improved cancer cell targeting with a stronger affinity to U87MG glioma cells, which have a high expression of alpha(v)beta(3) integrins, but minimal binding to MCF-7 breast cancer cells with low expression of alpha(v)beta(3) integrins.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-10342385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-11457171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-14531702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-14640638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-15232722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-15258594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-15681376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-15771523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-16618187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-16734494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-17308094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-17309256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-17319667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-17393074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-17891134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-17892287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-18193025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-18324840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19089838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19099431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19150737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19169455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19388661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19422261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19595457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19765817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19773081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19817050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-19835370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20398933-8860681
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1878-5905
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5397-407
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pubmed:dateRevised |
2011-8-1
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pubmed:meshHeading |
pubmed-meshheading:20398933-Animals,
pubmed-meshheading:20398933-Biofouling,
pubmed-meshheading:20398933-Cell Death,
pubmed-meshheading:20398933-Cell Line, Tumor,
pubmed-meshheading:20398933-Coated Materials, Biocompatible,
pubmed-meshheading:20398933-Culture Media,
pubmed-meshheading:20398933-Humans,
pubmed-meshheading:20398933-Iron,
pubmed-meshheading:20398933-Macrophages,
pubmed-meshheading:20398933-Methacrylates,
pubmed-meshheading:20398933-Mice,
pubmed-meshheading:20398933-Mononuclear Phagocyte System,
pubmed-meshheading:20398933-Nanoparticles,
pubmed-meshheading:20398933-Particle Size,
pubmed-meshheading:20398933-Polyethylene Glycols,
pubmed-meshheading:20398933-Quantum Dots,
pubmed-meshheading:20398933-Spectrometry, Fluorescence,
pubmed-meshheading:20398933-Staining and Labeling,
pubmed-meshheading:20398933-Tissue Distribution
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pubmed:year |
2010
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