Linked Life Data

20393024

Source:http://linkedlifedata.com/resource/pubmed/id/20393024

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-4-15
pubmed:abstractText
The present study determined mutations of phosphatase and tensin homolog (PTEN) gene in patients suffering from high-grade gliomas (WHO grades III and IV) and further investigated the mutations in correlation to patients' histopathological classification and short-term survival. Total RNA and genomic DNA were extracted from tumor tissues. Full-length PTEN cDNA sequences were amplified by polymerase chain reaction (PCR). The PCR products were directly sequenced, and the PTEN mutations were analyzed. It demonstrated that the incidence of PTEN mutations was 8/22 in these patients: one patient with WHO grade III glioma (1/11) and 7 patients with WHO grade IV glioma (7/11). Most patients had three or more mutations in the PTEN gene, with exons 2, 3, 4, 5, 6 and 7 as hot mutation regions, with mutation incidence from 62.5% to 75%. About 68.4% of mutations were missense, 26.3% same-sense and 5.3% nonsense mutations. The median survival times of the WHO grade III and IV groups were 250 and 53 weeks after surgery, respectively (p=0.016). The 36-week survival rate of patients with and without PTEN mutations was 62.5% and 92.9% (p=0.038, odds ratio=7.80), respectively. The present study suggests that PTEN mutations are late events in the malignant progression of glioma and the occurrence of PTEN mutations are significantly correlated to patients' short-term survival.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1791-7530
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
981-5
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Mutations of PTEN gene in gliomas correlate to tumor differentiation and short-term survival rate.
pubmed:affiliation
Section of Clinical Chemistry and Pharmacology, Institute of Laboratory Medicine, Lunds University, S-221 85 Lund, Sweden.
pubmed:publicationType
Journal Article