Source:http://linkedlifedata.com/resource/pubmed/id/20390928
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2010-4-14
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pubmed:abstractText |
The content and spectral characteristics of cytochrome P450 (Cyt P450) and cytochrome b(5) (Cyt b(5)) extracted from shoots of etiolated maize and rice seedlings were studied by using ultraviolet (UV) difference spectrophotometry. The results showed that fenclorim, rac-metolachlor and S-metolachlor may induce the same P450 isoenzyme with lambda(max) at 453 nm, while naphthalic anhydride (NA) induced another one with lambda(max) at 447 nm. The microsomal Cyt P450 and Cyt b(5) content of maize seedlings was higher than that of rice, and the Cyt b(5) content was higher than that of Cyt P450. Maize and rice microsomal Cyt P450 and Cyt b(5) were induced at different levels by the four chemicals, with the order as follows: NA > fenclorim > rac-metolachlor > S-metolachlor with p < 0.05. When induced by NA, fenclorim, rac-metolachlor and S-metolachlor, the maize Cyt P450 content was, respectively, 5.63-, 3.30-, 3.02- and 2.48-fold that of the control, the rice Cyt P450 content was 8.54-, 2.20-, 1.91- and 1.33-fold that of the control, the maize Cyt b(5) content was 9.89-, 5.49-, 4.69- and 3.40-fold that of the control, and the rice Cyt b(5) content was 7.76-, 4.56-, 2.60- and 1.82-fold that of the control. An enantio-difference existed when rac- and S-metolachlor combined with plant Cyt P450. The interaction of microsomal Cyt P450 with S-metolachlor is higher than that with rac-metolachlor, which may be one of the reasons why S-metolachlor is superior at killing weeds compared with rac-metolachlor. These results will help to develop an understanding of the tolerance for and selectivity of rac- and S-metolachlor.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetamides,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes b5,
http://linkedlifedata.com/resource/pubmed/chemical/Herbicides,
http://linkedlifedata.com/resource/pubmed/chemical/alachlor
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1532-4109
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33-9
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pubmed:meshHeading |
pubmed-meshheading:20390928-Acetamides,
pubmed-meshheading:20390928-Cytochrome P-450 Enzyme System,
pubmed-meshheading:20390928-Cytochromes b5,
pubmed-meshheading:20390928-Enzyme Induction,
pubmed-meshheading:20390928-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:20390928-Gene Expression Regulation, Plant,
pubmed-meshheading:20390928-Herbicides,
pubmed-meshheading:20390928-Oryza sativa,
pubmed-meshheading:20390928-Spectrophotometry, Ultraviolet,
pubmed-meshheading:20390928-Zea mays
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pubmed:year |
2010
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pubmed:articleTitle |
Spectral characterization and chiral interactions of plant microsomal cytochrome P450 with metolachlor and herbicide safeners.
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pubmed:affiliation |
College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, People's Republic of China. lhj@mail.zjgsu.edu.cn
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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