|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2010-4-16
|
|
pubmed:abstractText |
hsa-mir-483 is located within intron 2 of the IGF2 locus. We found that the mature microRNA (miRNA) miR-483-3p is overexpressed in 100% of Wilms' tumors. In addition, colon, breast, and liver cancers exhibit high or even extremely high levels of miR-483-3p in approximately 30% of the cases. A coregulation with IGF2 mRNA was detected, although some tumors exhibited high expression of miR-483-3p without a concomitant increase of IGF2. These findings suggested that miR-483-3p could cooperate with IGF2 or act as an autonomous oncogene. Indeed, here we prove that an anti-miRNA oligonucleotide against miR-483-3p could inhibit the miRNAs without affecting IGF2 mRNA and it could suppress tumorigenicity of HepG2 cells, a cell line that overexpresses miR-483-3p and IGF2. Conversely, no antitumor effect was elicited by inhibition of IGF2. The oncogenic mechanism of miR-483-3p was at least partially clarified by the finding that it could modulate the proapoptotic protein BBC3/PUMA and miR-483-3p enforced expression could protect cells from apoptosis. Our results indicate that miR-483-3p could function as an antiapoptotic oncogene in various human cancers and reveal a new, potentially important target for anticancer therapy.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
1538-7445
|
|
pubmed:author |
pubmed-author:AlderHansjuergH,
pubmed-author:AngioniAdrianoA,
pubmed-author:BolondiLuigiL,
pubmed-author:ConsiglioJessicaJ,
pubmed-author:CroceCarlo MCM,
pubmed-author:D'EliaGemmaG,
pubmed-author:FerracinManuelaM,
pubmed-author:FornariFrancescaF,
pubmed-author:GramantieriLauraL,
pubmed-author:LanzaGiovanniG,
pubmed-author:LupiniLauraL,
pubmed-author:NegriniMassimoM,
pubmed-author:QuerzoliPatriziaP,
pubmed-author:VeroneseAngeloA,
pubmed-author:VisoneRosaR,
pubmed-author:ZanesiNicolaN
|
|
pubmed:copyrightInfo |
(c) 2010 AACR.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
15
|
|
pubmed:volume |
70
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3140-9
|
|
pubmed:meshHeading |
pubmed-meshheading:20388800-Animals,
pubmed-meshheading:20388800-Apoptosis,
pubmed-meshheading:20388800-Cell Line, Tumor,
pubmed-meshheading:20388800-Hep G2 Cells,
pubmed-meshheading:20388800-Humans,
pubmed-meshheading:20388800-Insulin-Like Growth Factor II,
pubmed-meshheading:20388800-Introns,
pubmed-meshheading:20388800-Mice,
pubmed-meshheading:20388800-Mice, SCID,
pubmed-meshheading:20388800-MicroRNAs,
pubmed-meshheading:20388800-Oligonucleotides,
pubmed-meshheading:20388800-Oncogenes,
pubmed-meshheading:20388800-RNA, Messenger,
pubmed-meshheading:20388800-RNA, Small Interfering,
pubmed-meshheading:20388800-Wilms Tumor
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Oncogenic role of miR-483-3p at the IGF2/483 locus.
|
|
pubmed:affiliation |
Dipartimento di Medicina Sperimentale e Diagnostica, Università di Ferrara, Ferrara, Italy.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
