Linked Life Data

20386348

Source:http://linkedlifedata.com/resource/pubmed/id/20386348

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-4-16
pubmed:abstractText
Embryonic neural stem cells (NSCs) were isolated from the neuroepithelium of the dorsal telencephalon of embryonic rats and infected by Ad5-Atoh1-enhanced green fluorescent protein. These NSCs were then delivered into neurosphere culture medium or transplanted into the endolymphatic space of the normal guinea pig cochlea through cochleostomy. Embryonic NSC phenotype of these isolated cells was determined by immunohistochemical detection of cell-specific protein markers. Survival, location and hair cell (HC) differentiation of the implanted NSCs were determined by the expression of the report gene, enhanced green fluorescent protein, and a specific marker for HCs, Myosin VIIa. These implanted cells can survive in the endolymphatic space of the cochlea. Some of the surviving cells differentiated into HCs by Atoh1 gene transfer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1473-558X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
12
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
490-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Survival and fate of transplanted embryonic neural stem cells by Atoh1 gene transfer in guinea pigs cochlea.
pubmed:affiliation
Department of Otorhinolaryngology bCentral Laboratory, Eye and ENT Hospital, Fudan University, Shanghai, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't