Source:http://linkedlifedata.com/resource/pubmed/id/20385759
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2010-5-20
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pubmed:abstractText |
Strain AM-19226 is a pathogenic non-O1/non-O139 serogroup Vibrio cholerae strain that does not encode the toxin-coregulated pilus or cholera toxin but instead causes disease using a type three secretion system (T3SS). Two genes within the T3SS pathogenicity island, herein named vttR(A) (locus tag A33_1664) and vttR(B) (locus tag A33_1675), are predicted to encode proteins that show similarity to the transcriptional regulator ToxR, which is found in all strains of V. cholerae. Strains with a deletion of vttR(A) or vttR(B) showed attenuated colonization in vivo, indicating that the T3SS-encoded regulatory proteins play a role in virulence. lacZ transcriptional reporter fusions to intergenic regions upstream of genes encoding the T3SS structural components identified growth in the presence of bile as a condition that modulates gene expression. Under this condition, VttR(A) and VttR(B) were necessary for maximal gene expression. In contrast, growth in bile did not substantially alter the expression of a reporter fusion to the vopF gene, which encodes an effector protein. Increased vttR(B) reporter fusion activity was observed in a DeltavttR(B) strain background, suggesting that VttR(B) may regulate its own expression. The collective results are consistent with the hypothesis that T3SS-encoded regulatory proteins are essential for pathogenesis and control the expression of selected T3SS genes.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/toxR protein, Vibrio cholerae
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1098-5522
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2554-70
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pubmed:dateRevised |
2011-4-6
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pubmed:meshHeading |
pubmed-meshheading:20385759-Animals,
pubmed-meshheading:20385759-Anti-Bacterial Agents,
pubmed-meshheading:20385759-Artificial Gene Fusion,
pubmed-meshheading:20385759-Bacterial Proteins,
pubmed-meshheading:20385759-Bile,
pubmed-meshheading:20385759-DNA-Binding Proteins,
pubmed-meshheading:20385759-Gene Deletion,
pubmed-meshheading:20385759-Gene Expression Regulation, Bacterial,
pubmed-meshheading:20385759-Genes, Reporter,
pubmed-meshheading:20385759-Mice,
pubmed-meshheading:20385759-Stress, Physiological,
pubmed-meshheading:20385759-Transcription Factors,
pubmed-meshheading:20385759-Vibrio cholerae non-O1,
pubmed-meshheading:20385759-Virulence,
pubmed-meshheading:20385759-beta-Galactosidase
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pubmed:year |
2010
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pubmed:articleTitle |
vttRA and vttRB Encode ToxR family proteins that mediate bile-induced expression of type three secretion system genes in a non-O1/non-O139 Vibrio cholerae strain.
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pubmed:affiliation |
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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