Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-6-10
pubmed:abstractText
Because both iron deficiency and iron excess are deleterious to normal cell function, the intracellular level of iron must be tightly controlled. Ferritin, an iron binding protein, regulates iron balance by storing iron in a bioavailable but nontoxic form. Ferritin protein comprises two subunits: ferritin H, which contains ferroxidase activity, and ferritin L. Here we demonstrate that ferritin H mRNA and protein are induced by histone deacetylase inhibitors (HDAC inhibitors), a promising class of anti-cancer drugs, in cultured human cancer cells. Deletion analysis and EMSA assays reveal that the induction of ferritin H occurs at a transcriptional level via Sp1 and NF-Y binding sites near the transcriptional start site of the human ferritin H promoter. Classically, HDAC inhibitors modulate gene expression by increasing histone acetylation. However, ChIP assays demonstrate that HDAC inhibitors induce ferritin H transcription by increasing NF-Y binding to the ferritin H promoter without changes in histone acetylation. These results identify ferritin H as a new target of HDAC inhibitors, and recruitment of NF-Y as a novel mechanism of action of HDAC inhibitors.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-10066817, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-10075648, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-10409740, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-10485488, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-10652280, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-10667218, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-10833524, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-10922406, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-11034398, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-11415455, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-11877441, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-11902574, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-11980894, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-11986201, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-12151407, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-12176973, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-12435735, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-12511565, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-12750289, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-12832471, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-12892709, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-14586402, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-14651963, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15107822, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15109490, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15169878, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15251435, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15313420, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15352027, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15369676, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-1541403, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15637150, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-15722551, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-16088937, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-16186112, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-16545682, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-17604281, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-17894944, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-18628067, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-18815279, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-18819919, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-2481317, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-8634457, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-9032265, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-9252395, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-9311776, http://linkedlifedata.com/resource/pubmed/commentcorrection/20385107-9632821
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1873-2968
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
316-24
pubmed:dateRevised
2011-8-3
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Ferritin H induction by histone deacetylase inhibitors.
pubmed:affiliation
Department of Cancer Biology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, United States.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural