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rdf:type |
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lifeskim:mentions |
umls-concept:C0029246,
umls-concept:C0035687,
umls-concept:C0035696,
umls-concept:C0079411,
umls-concept:C0083032,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0150312,
umls-concept:C0332307,
umls-concept:C0443199,
umls-concept:C0591833,
umls-concept:C1335671
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pubmed:issue |
6
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pubmed:dateCreated |
1991-7-3
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pubmed:abstractText |
To better understand the regulation of interleukin-7 receptor (IL-7R) expression, we have pursued a detailed analysis of the structure of the murine and human IL-7R genes. The genes consist of eight exons, the sizes of which are conserved in mouse and human cells, spread out over 24 kbp (murine) and 19 kbp (human). A differential splicing event results in an mRNA encoding a secreted form of the human IL-7R gene. Primer extension and S1 nuclease analysis show a single transcriptional start site for the murine IL-7R gene. The 5'-flanking region of the murine IL-7R gene contains TATA- and CAAT-like sequences. The promoter region also contains a functional interferon regulatory element, to which the interferon-induced nuclear factors IRF-1 and IRF-2 are capable of binding and which is able to confer interferon-inducible expression on a heterologous gene. There are also potential binding sites for the transcription factors AP-1 and AP-2 as well as multiple glucocorticoid response elements. A fusion gene containing 2.5 kb of murine IL-7R 5' regulatory sequence linked to the bacterial chloramphenicol acetyltransferase gene directed expression of chloramphenicol acetyltransferase activity in murine pre-B-cell line 70Z/3 but not in the mouse fibroblast cell line NIH 3T3. Comparison of the murine and human IL-7R exon/intron boundaries with those of other hematopoietin receptor superfamily members whose exon/intron boundaries are also known reveals a conserved evolutionary structure.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2038316-1973832,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2038316-922889
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3052-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2038316-Humans,
pubmed-meshheading:2038316-Animals,
pubmed-meshheading:2038316-Mice,
pubmed-meshheading:2038316-Pregnancy,
pubmed-meshheading:2038316-Liver,
pubmed-meshheading:2038316-Placenta,
pubmed-meshheading:2038316-Female,
pubmed-meshheading:2038316-Base Sequence,
pubmed-meshheading:2038316-Protein Biosynthesis,
pubmed-meshheading:2038316-RNA, Messenger,
pubmed-meshheading:2038316-Amino Acid Sequence,
pubmed-meshheading:2038316-Molecular Sequence Data
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