Source:http://linkedlifedata.com/resource/pubmed/id/20383134
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2010-4-30
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pubmed:abstractText |
Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1548-7105
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
395-7
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pubmed:meshHeading |
pubmed-meshheading:20383134-Actins,
pubmed-meshheading:20383134-Animals,
pubmed-meshheading:20383134-Cell Line, Tumor,
pubmed-meshheading:20383134-Cells, Cultured,
pubmed-meshheading:20383134-Humans,
pubmed-meshheading:20383134-Mice,
pubmed-meshheading:20383134-Nucleic Acid Amplification Techniques,
pubmed-meshheading:20383134-Point Mutation,
pubmed-meshheading:20383134-RNA, Messenger,
pubmed-meshheading:20383134-Receptor, erbB-2,
pubmed-meshheading:20383134-Transcription, Genetic
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pubmed:year |
2010
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pubmed:articleTitle |
In situ detection and genotyping of individual mRNA molecules.
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pubmed:affiliation |
Department of Genetics and Pathology, Uppsala University, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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