Linked Life Data

20378691

Source:http://linkedlifedata.com/resource/pubmed/id/20378691

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-5-31
pubmed:abstractText
In this study, a group of 313 workers occupationally exposed to vinyl chloride monomer (VCM) and 141 normal unexposed referents were examined for chromosomal damage using the cytokinesis-blocked micronucleus (CBMN) assay in peripheral lymphocytes. We explored the relationship between genetic polymorphisms of XRCC1 (Arg194Trp, Arg280His and Arg399Gln), MGMT(Leu84Phe) and hOGG1 (Ser326Cys) and susceptibility of chromosomal damage induced by VCM. Polymerase chain reaction-restriction fragment length polymorphism techniques were used to detect polymorphisms in XRCC1, hOGG1 and MGMT. It was found that the micronuclei (MN) frequency of exposed workers (4.86 +/- 2.80) per thousand was higher than that of the control group (1.22 +/- 1.24) per thousand (P < 0.01). Increased susceptibility to chromosomal damage as evidenced by higher MN frequency was found in workers with hOGG1 326 Ser/Cys genotype [frequency ratio (FR) = 1.21, 95% confidence interval (CI): 1.02-1.46; P < 0.05], XRCC1 194 Arg/Trp (FR = 1.12, 95% CI: 1.00-1.25; P < 0.05) and XRCC1 280 Arg/His and His/His genotypes (FR = 1.12, 95% CI 1.00-1.26, P < 0.05). Moreover, among susceptibility diplotypes, CGA/CAG carriers had more risk of MN frequency compared with individuals with wild-type CGG/CGG (FR = 1.67, 95% CI: 1.19-2.23; P < 0.05). MN frequency also increased significantly with age in the exposed group (FR = 1.13, 95% CI: 1.00-1.28; P < 0.05). Thus, CB-MN was a sensitive index of early damage among VCM-exposed workers. Genotype XRCC1 Arg194Trp, Arg280His, hOGG1 Ser326Cys, diplotype CGA/CAG and higher age may have an impact on the chromosome damage induced by VCM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1460-2180
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1068-73
pubmed:meshHeading
pubmed-meshheading:20378691-Adult, pubmed-meshheading:20378691-Base Sequence, pubmed-meshheading:20378691-China, pubmed-meshheading:20378691-DNA Glycosylases, pubmed-meshheading:20378691-DNA Modification Methylases, pubmed-meshheading:20378691-DNA Primers, pubmed-meshheading:20378691-DNA Repair Enzymes, pubmed-meshheading:20378691-DNA-Binding Proteins, pubmed-meshheading:20378691-Female, pubmed-meshheading:20378691-Humans, pubmed-meshheading:20378691-Life Style, pubmed-meshheading:20378691-Male, pubmed-meshheading:20378691-Micronucleus Tests, pubmed-meshheading:20378691-Middle Aged, pubmed-meshheading:20378691-Occupational Exposure, pubmed-meshheading:20378691-Poisson Distribution, pubmed-meshheading:20378691-Polymorphism, Genetic, pubmed-meshheading:20378691-Tumor Suppressor Proteins, pubmed-meshheading:20378691-Vinyl Chloride
pubmed:year
2010
pubmed:articleTitle
Genetic polymorphisms of XRCC1, HOGG1 and MGMT and micronucleus occurrence in Chinese vinyl chloride-exposed workers.
pubmed:affiliation
Department of Occupational Health and Toxicology, School of Public Health, Fudan University, and Key laboratory of public health and safety of Ministry of Education of China, 138 Yixue yuan Road, Shanghai, 200032,China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't