|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2010-9-9
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 microg AHL h(-1) per 10(9) CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif (106)HXDH-59 amino acids-H(169)-21 amino acids-D(191) for N-acylhomoserine lactone lactonases.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
1572-9699
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
98
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
299-305
|
|
pubmed:meshHeading |
pubmed-meshheading:20376561-4-Butyrolactone,
pubmed-meshheading:20376561-Acyl-Butyrolactones,
pubmed-meshheading:20376561-Amino Acid Sequence,
pubmed-meshheading:20376561-Bacillus cereus,
pubmed-meshheading:20376561-Bacterial Proteins,
pubmed-meshheading:20376561-Carboxylic Ester Hydrolases,
pubmed-meshheading:20376561-Chromobacterium,
pubmed-meshheading:20376561-Cloning, Molecular,
pubmed-meshheading:20376561-DNA, Bacterial,
pubmed-meshheading:20376561-Escherichia coli,
pubmed-meshheading:20376561-Genes, Bacterial,
pubmed-meshheading:20376561-Gram-Negative Bacteria,
pubmed-meshheading:20376561-Malaysia,
pubmed-meshheading:20376561-Molecular Sequence Data,
pubmed-meshheading:20376561-Quorum Sensing,
pubmed-meshheading:20376561-Sequence Analysis, DNA,
pubmed-meshheading:20376561-Soil Microbiology,
pubmed-meshheading:20376561-Trees
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil.
|
|
pubmed:affiliation |
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. kokgan@um.edu.my
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
