Linked Life Data

20376101

Source:http://linkedlifedata.com/resource/pubmed/id/20376101

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2010-8-24
pubmed:abstractText
Petalomonas sphagnophila is a poorly studied plastid-lacking euglenid flagellate living in Sphagnum-dominated peatlands. Here we present a broad-ranging microscopic, molecular and microspectrophotometric analysis of uncultured P. sphagnophila collected from four field locations in Nova Scotia, Canada. Consistent with its morphological characteristics, 18S ribosomal DNA (rDNA) phylogenies indicate that P. sphagnophila is specifically related to Petalomonas cantuscygni, the only other Petalomonas species sequenced to date. One of the peculiar characteristics of P. sphagnophila is the presence of several green-pigmented particles approximately 5 mum in diameter in its cytoplasm, which a previously published study suggested to be cyanobacterial endosymbionts. New data presented here, however, suggest that the green intracellular body may not be a cyanobacterium but rather an uncharacterized prokaryote yet to be identified by molecular sequencing. 16S rDNA library sequencing and fluorescence in situ hybridizations show that P. sphagnophila also harbors several other endobionts, including bacteria that represent five novel genus-level groups (one firmicute and four different proteobacteria). 16S rDNA phylogenies suggest that three of these endobionts are related to obligate intracellular bacteria such as Rickettsiales and Coxiella, while the others are related to the Daphnia pathogen Spirobacillus cienkowskii or belong to the Thermoactinomycetaceae. TEM, 16S rDNA library sequencing and a battery of PCR experiments show that the presence of the five P. sphagnophila endobionts varies markedly among the four geographic collections and even among individuals collected from the same location but at different time points. Our study adds significantly to the growing evidence for complex and dynamic protist-bacterial associations in nature.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1751-7370
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1108-20
pubmed:meshHeading
pubmed-meshheading:20376101-Bacteria, pubmed-meshheading:20376101-Cluster Analysis, pubmed-meshheading:20376101-Cytoplasm, pubmed-meshheading:20376101-DNA, Bacterial, pubmed-meshheading:20376101-DNA, Protozoan, pubmed-meshheading:20376101-DNA, Ribosomal, pubmed-meshheading:20376101-Euglenida, pubmed-meshheading:20376101-Microscopy, pubmed-meshheading:20376101-Microscopy, Electron, Transmission, pubmed-meshheading:20376101-Microscopy, Fluorescence, pubmed-meshheading:20376101-Molecular Sequence Data, pubmed-meshheading:20376101-Nova Scotia, pubmed-meshheading:20376101-Phylogeny, pubmed-meshheading:20376101-RNA, Ribosomal, 16S, pubmed-meshheading:20376101-RNA, Ribosomal, 18S, pubmed-meshheading:20376101-Sequence Analysis, DNA, pubmed-meshheading:20376101-Soil, pubmed-meshheading:20376101-Soil Microbiology, pubmed-meshheading:20376101-Symbiosis
pubmed:year
2010
pubmed:articleTitle
Complex array of endobionts in Petalomonas sphagnophila, a large heterotrophic euglenid protist from Sphagnum-dominated peatlands.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Canadian Institute for Advanced Research, Program in Integrated Microbial Biodiversity, Dalhousie University, Halifax, Nova Scotia, Canada. eunsookim@dal.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't