Linked Life Data

20374877

Source:http://linkedlifedata.com/resource/pubmed/id/20374877

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-4-8
pubmed:abstractText
A mathematical model of anaerobic muscle energy-metabolism was developed to predict pH and the concentrations of nine muscle metabolites over time. Phosphorous-31 Nuclear Magnetic Resonance was used to measure time-course data for some phosphate metabolites and pH in anoxic M. semitendinosus taken from three slaughtered sheep. Muscles were held at 35 degrees C during the experiment. Measurement commenced 25 min post mortem and concluded before rigor mortis. The model was fitted to these data within experimental error, by simultaneously varying model parameter values and initial substrate concentrations. The model was used to simulate the period from death until metabolic activity ceased, in order to predict the different stages of metabolic response to anoxia. The model suggested that alkalinisation would occur in all three muscles in the first few minutes after the onset of anoxia, followed by a steady decline in pH. For two of the muscles this decline continued until rigor, with final pH values of 5.60 and 6.07. For the other muscle, pH reached a low of 5.60 near rigor but then increased to a final value of 5.73. A rise in pH after rigor has been observed but not previously explained in the literature. The modelling results suggest it was caused by the alkalising effect of adenosine monophosphate deamination being greater at low pH than the acidifying effect of inosine monophosphate dephosphorylation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1873-4138
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2009 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
134-48
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Modelling muscle energy-metabolism in anaerobic muscle.
pubmed:affiliation
AgResearch Limited, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand. kumar.vetharaniam@agresearch.co.nz
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't