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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2010-7-19
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pubmed:abstractText |
1. Serotonin (5-hydroxytryptamine; 5-HT) plays important roles in the development of cardiac hypertrophy via activation of 5-HT receptors. The aim of the present study was to investigate the role of 5-HT(2B) receptors in the development of cardiomyocyte apoptosis and hypertrophy associated with noradrenaline (NA) overload. 2. Cardiac hypertrophy was induced in rats by intraperitoneal injection of 1.5 mg/kg NA for 4 weeks. Starting from Day 15, 5-HT2B receptor antagonist SB 204741 (i.p., 0.5 or 2 mg/kg) or SDZ SER 082 (i.p., 1 mg/kg) was injected twice daily for another 14 days. Whole-cell patch-clamp techniques were used to record ionic currents in freshly isolated ventricular cardiomyocytes. Western blot and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) assays were used to assess myocardial apoptosis. 3. Expression of 5-HT(2B) receptors was enhanced in the hypertrophic left ventricle induced by NE overload in vivo. The 5-HT(2B) receptor antagonist SB 204741 partially reversed cardiac hypertrophy induced by NE overload (P < 0.05) and decreased L-type calcium currents in ventricular cardiomyocytes (P < 0.05). In addition, SB 204741 notably attenuated myocardial apoptosis, as evidenced by downregulation of Bax and caspase 3 (P < 0.05) and upregulation of the anti-apoptotic Bcl-2 protein (P < 0.05). 4. In conclusion, the data suggest an involvement of 5-HT(2B) receptors in the generation of apoptotic events associated with cardiac remodelling during increased adrenergic stimulation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,5,7a,8,9,10,11,11a-octohydro-7H-10...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, L-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/N-(1-methyl-5-indolyl)-N'-(3-methyl-...,
http://linkedlifedata.com/resource/pubmed/chemical/Naphthyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Serotonin, 5-HT2B,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin 5-HT2 Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Urea,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1440-1681
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
e145-51
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:20374255-Animals,
pubmed-meshheading:20374255-Apoptosis,
pubmed-meshheading:20374255-Calcium Channels, L-Type,
pubmed-meshheading:20374255-Cardiomegaly,
pubmed-meshheading:20374255-Cardiomyopathies,
pubmed-meshheading:20374255-Caspase 3,
pubmed-meshheading:20374255-Down-Regulation,
pubmed-meshheading:20374255-Indoles,
pubmed-meshheading:20374255-Myocytes, Cardiac,
pubmed-meshheading:20374255-Naphthyridines,
pubmed-meshheading:20374255-Norepinephrine,
pubmed-meshheading:20374255-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:20374255-Rats,
pubmed-meshheading:20374255-Rats, Sprague-Dawley,
pubmed-meshheading:20374255-Receptor, Serotonin, 5-HT2B,
pubmed-meshheading:20374255-Serotonin,
pubmed-meshheading:20374255-Serotonin 5-HT2 Receptor Antagonists,
pubmed-meshheading:20374255-Up-Regulation,
pubmed-meshheading:20374255-Urea,
pubmed-meshheading:20374255-bcl-2-Associated X Protein
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pubmed:year |
2010
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pubmed:articleTitle |
Role of 5-HT 2B receptors in cardiomyocyte apoptosis in noradrenaline-induced cardiomyopathy in rats.
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pubmed:affiliation |
Department of Cardiac Surgery, Xijing Hospital, Xi'an, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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