Linked Life Data

20373497

Source:http://linkedlifedata.com/resource/pubmed/id/20373497

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-4-7
pubmed:abstractText
In studies of eukaryotic cell cycle regulation, the budding yeast Saccharoymyces cerevisiae offers many advantages as a model system. Due to its simple growth requirements and genetic tractability, this organism is a powerful tool for investigating the molecular regulation of cell cycle control. One earlier disadvantage to performing cell cycle analyses in yeast was that existing methods were restricted to either visual analysis or flow cytometry, both of which present limitations in the scope and accuracy of the data obtained. This unit demonstrates the combined use of DNA content measurements and bright-field image analysis using multispectral imaging flow cytometry (MIFC) to provide a more precise quantitation of yeast cell cycle distribution. The advantage of this method is the ability to analyze large numbers of cells using multiple cell cycle indicators in a relatively short amount of time.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1934-9300
pubmed:author
pubmed:copyrightInfo
(c) 2010 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 9.32.1-16
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Yeast cell cycle analysis: combining DNA staining with cell and nuclear morphology.
pubmed:affiliation
Temasek Life Sciences Laboratory, National University of Singapore, Singapore.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural