Source:http://linkedlifedata.com/resource/pubmed/id/20372819
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2010-4-7
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| pubmed:abstractText |
Pinus massoniana bark extract (PMBE) is a mixture of flavonoids, whose antioxidant and apoptosis-inducing properties have been confirmed in vitro. In this study, the apoptotic effect and mechanism of PMBE in HepG2 human hepatoma cells were evaluated. PMBE exerted dose- and time-dependent cell growth inhibition on HepG2 cells, and selectively induced apoptosis without impact on normal liver L-02 cells. Apoptosis induced by PMBE in HepG2 cells was also confirmed by annexin-V/PI staining, transmission electron microscopy and sub-G1 phase accumulation. Moreover, PMBE also slightly blocked the cell cycle in the G2/M and S phases in HepG2 cells. The investigation of the mechanism by which PMBE induced apoptosis in HepG2 cells indicated that activation of extrinsic and intrinsic caspase, inhibition of NF-kappaB activation and decrease of the antiapoptotic protein Bcl-2 and the intact Bid protein were involved. Furthermore, the antitumor activity of PBME was demonstrated in vivo by a 42.88-69.94% reduction rate of tumor weight in H22 tumor-implanted mice. Taken together, these data indicate that PMBE selectively induces apoptosis in HepG2 cells through caspase-dependent pathways, and inhibits tumor growth in vivo, making it a potential candidate for anticancer therapeutics.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Drugs, Chinese Herbal,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Proanthocyanidins
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1791-244X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
25
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
751-9
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| pubmed:meshHeading |
pubmed-meshheading:20372819-Animals,
pubmed-meshheading:20372819-Antioxidants,
pubmed-meshheading:20372819-Apoptosis,
pubmed-meshheading:20372819-Carcinoma, Hepatocellular,
pubmed-meshheading:20372819-Caspases,
pubmed-meshheading:20372819-Cell Cycle,
pubmed-meshheading:20372819-Cell Line,
pubmed-meshheading:20372819-Cell Line, Tumor,
pubmed-meshheading:20372819-Drug Screening Assays, Antitumor,
pubmed-meshheading:20372819-Drugs, Chinese Herbal,
pubmed-meshheading:20372819-Humans,
pubmed-meshheading:20372819-Liver Neoplasms,
pubmed-meshheading:20372819-Medicine, Chinese Traditional,
pubmed-meshheading:20372819-Mice,
pubmed-meshheading:20372819-Neoplasm Transplantation,
pubmed-meshheading:20372819-Phytotherapy,
pubmed-meshheading:20372819-Pinus,
pubmed-meshheading:20372819-Plant Bark,
pubmed-meshheading:20372819-Plant Extracts,
pubmed-meshheading:20372819-Proanthocyanidins
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| pubmed:year |
2010
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| pubmed:articleTitle |
Pinus massoniana bark extract selectively induces apoptosis in human hepatoma cells, possibly through caspase-dependent pathways.
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| pubmed:affiliation |
The State Key Laboratory of Biocontrol and The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen Zhongshan University, Guangzhou 510275, Guangdong Province, PR China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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