pubmed-article:2037014 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2037014 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
pubmed-article:2037014 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
pubmed-article:2037014 | lifeskim:mentions | umls-concept:C0023206 | lld:lifeskim |
pubmed-article:2037014 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
pubmed-article:2037014 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
pubmed-article:2037014 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
pubmed-article:2037014 | pubmed:issue | 5 | lld:pubmed |
pubmed-article:2037014 | pubmed:dateCreated | 1991-6-28 | lld:pubmed |
pubmed-article:2037014 | pubmed:abstractText | Succinylation of concanavalin A (Con A) reduces its oligomer size while retaining its mitogenicity, and provides a probe of T cell activation. We have observed responses of cytosolic ionized calcium to succinyl Con A in suspensions of Jurkat and rat lymph node (LN) cells, using a fluorimeter, and in single cells settled on glass, using a dual wavelength video imaging system. In the fluorimeter a mitogenic level of succinyl Con A (30 micrograms/ml) produced only a 15-30 nM rise in average cell calcium in the suspended Jurkat or rat cells whereas the use of quantitative video imaging produced asynchronous 250-1000 nM pulses of free calcium in 35% of Jurkat cells and 300-850 nM pulses in 45% of rat LN cells. In Jurkat cells these pulses were sometimes repetitive, giving rise to apparent oscillations. In the fluorimeter 30 micrograms/ml of native Con A (a supra-mitogenic concentration) produced a 300 nM rise in average cell calcium in suspended Jurkat cells, and a 100 nM rise in rat LN cells; when major histocompatibility complex class II-bearing cells were removed the response rose. Mitogenic Con A (3 micrograms/ml) produced a much lower rise in calcium. With video imaging the response seen was greater. Levels greater than 30 micrograms/ml Con A caused 700-5000 nM pulses synchronously in 94% of Jurkat cells and 250-1000 nM pulses in 73% of rat LN cells. At 3 micrograms/ml Con A produced asynchronous 300-1100 nM pulses in 36% of rat LN cells. We conclude that the absence of a calcium signal in the fluorimeter can conceal asynchronous calcium responses in individual cells and that brief asynchronous cytosolic calcium pulses are sufficient for lectin to activate rat T cells. | lld:pubmed |
pubmed-article:2037014 | pubmed:language | eng | lld:pubmed |
pubmed-article:2037014 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2037014 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:2037014 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2037014 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2037014 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2037014 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2037014 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2037014 | pubmed:month | May | lld:pubmed |
pubmed-article:2037014 | pubmed:issn | 0014-2980 | lld:pubmed |
pubmed-article:2037014 | pubmed:author | pubmed-author:TregearR TRT | lld:pubmed |
pubmed-article:2037014 | pubmed:author | pubmed-author:MasonW TWT | lld:pubmed |
pubmed-article:2037014 | pubmed:author | pubmed-author:HoylandJJ | lld:pubmed |
pubmed-article:2037014 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2037014 | pubmed:volume | 21 | lld:pubmed |
pubmed-article:2037014 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2037014 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2037014 | pubmed:pagination | 1283-8 | lld:pubmed |
pubmed-article:2037014 | pubmed:dateRevised | 2003-11-14 | lld:pubmed |
pubmed-article:2037014 | pubmed:meshHeading | pubmed-meshheading:2037014-... | lld:pubmed |
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pubmed-article:2037014 | pubmed:meshHeading | pubmed-meshheading:2037014-... | lld:pubmed |
pubmed-article:2037014 | pubmed:year | 1991 | lld:pubmed |
pubmed-article:2037014 | pubmed:articleTitle | Calcium signals in single T cells on activation by lectin. | lld:pubmed |
pubmed-article:2037014 | pubmed:affiliation | AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, GB. | lld:pubmed |
pubmed-article:2037014 | pubmed:publicationType | Journal Article | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2037014 | lld:pubmed |