Linked Life Data

20369214

Source:http://linkedlifedata.com/resource/pubmed/id/20369214

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-6-23
pubmed:abstractText
We demonstrate a new and extremely inexpensive, multipurpose desktop system for operating lab-on-a-chip (LOC) devices. The system provides all of the infrastructure necessary for genetic amplification and analysis, with orders of magnitude improvement in performance over our previous work. A modular design enables high levels of integration while allowing scalability to lower cost and smaller size. The component cost of this system is ca. $600, yet it could support many diagnostic applications. We demonstrate an implementation of genetic amplification via polymerase chain reaction (PCR), and analysis using capillary electrophoresis (CE). The PCR is able to amplify from single or several copies of target DNA and the CE performance (e.g. sensitivity) is comparable to that of commercial photomultiplier-based confocal lab-on-chip instrumentation. We believe this demonstrates that the cost of infrastructure need no longer be a barrier to the wide-spread application of LOC technologies in healthcare and beyond.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1364-5528
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1606-17
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
A scalable and modular lab-on-a-chip genetic analysis instrument.
pubmed:affiliation
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, Canada T6G 2V4.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't