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rdf:type |
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lifeskim:mentions |
umls-concept:C0018270,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0030956,
umls-concept:C0079731,
umls-concept:C0179302,
umls-concept:C0591833,
umls-concept:C0599894,
umls-concept:C1420799,
umls-concept:C1515655,
umls-concept:C1706253
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pubmed:issue |
2
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pubmed:dateCreated |
2010-7-16
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pubmed:abstractText |
B-cell lymphoma is a clonal expansion of neoplastic cells that may result in fatal outcomes. Here, we report the in vivo targeting and growth inhibition of aggressive A20 murine B-cell lymphoma by idiotype-specific peptide pA20-36. pA20-36 was selected from random peptide libraries and bound specifically to the B-cell receptor (BCR) of A20 cells in mice engrafted with A20 lymphoma, as shown by histology and positron emission tomographic analysis. BCR cross-linking of A20 cells with pA20-36 resulted in massive apoptosis of targeted tumor cells and in an increased survival of the diseased animals without any detectable evidence of toxicity. The pA20-36 treatment reverted the immune suppression of the tumor microenvironment as shown by reduced expression of vascular endothelial growth factor, interleukin-10, and transforming growth factor-beta cytokines together with a lower number of CD11b+Gr-1+ inhibitor myeloid-derived suppressor cells and Foxp3+CD4+ Treg cells. Furthermore, pA20-36 treatment was associated with an increased number of tumor-infiltrating, activated CD8+ T cells that exerted a tumor-specific cytolytic activity. These findings show that a short peptide that binds specifically to the complementarity-determining regions of the A20 BCR allows in vivo detection of neoplastic cells together with significant inhibition of tumor growth in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Idiotypes,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Library,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1528-0020
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pubmed:author |
pubmed-author:ArraClaudioC,
pubmed-author:BarbieriAntonioA,
pubmed-author:CostaNicolaN,
pubmed-author:Dal PiazFabrizioF,
pubmed-author:De LaurentiisAnnamariaA,
pubmed-author:FalconeCristinaC,
pubmed-author:FierroOlgaO,
pubmed-author:GargiuloSaraS,
pubmed-author:GaspariMarcoM,
pubmed-author:GiudiceAldoA,
pubmed-author:IaccinoEnricoE,
pubmed-author:LarobinaMicheleM,
pubmed-author:LubertoLauraL,
pubmed-author:PalmaGiuseppeG,
pubmed-author:PalmieriCamilloC,
pubmed-author:PanicoMaria RosariaMR,
pubmed-author:PisanoAntonioA,
pubmed-author:PontorieroMarilenaM,
pubmed-author:QuintoIleanaI,
pubmed-author:ScalaGiuseppeG,
pubmed-author:SchiavoneMarcoM,
pubmed-author:TrimboliFrancescaF,
pubmed-author:TuccilloFranca MariaFM,
pubmed-author:VecchioEleonoraE
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
226-38
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pubmed:meshHeading |
pubmed-meshheading:20363775-Animals,
pubmed-meshheading:20363775-Antibodies, Anti-Idiotypic,
pubmed-meshheading:20363775-Antibodies, Monoclonal,
pubmed-meshheading:20363775-Antibody Specificity,
pubmed-meshheading:20363775-Antineoplastic Agents,
pubmed-meshheading:20363775-Cell Line, Tumor,
pubmed-meshheading:20363775-Cell Proliferation,
pubmed-meshheading:20363775-Cell Separation,
pubmed-meshheading:20363775-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:20363775-Flow Cytometry,
pubmed-meshheading:20363775-Immunoglobulin Idiotypes,
pubmed-meshheading:20363775-Immunotherapy,
pubmed-meshheading:20363775-Lymphoma, B-Cell,
pubmed-meshheading:20363775-Mice,
pubmed-meshheading:20363775-Mice, Inbred BALB C,
pubmed-meshheading:20363775-Microscopy, Confocal,
pubmed-meshheading:20363775-Peptide Library,
pubmed-meshheading:20363775-Peptides,
pubmed-meshheading:20363775-Positron-Emission Tomography,
pubmed-meshheading:20363775-Receptors, Antigen, B-Cell,
pubmed-meshheading:20363775-Surface Plasmon Resonance
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pubmed:year |
2010
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pubmed:articleTitle |
In vivo targeting and growth inhibition of the A20 murine B-cell lymphoma by an idiotype-specific peptide binder.
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pubmed:affiliation |
Department of Experimental and Clinical Medicine, University of Catanzaro Magna Graecia, Catanzaro, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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